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Swim bladder collagen anti-oxidation and anti-fatigue protein peptide

A kind of swim bladder collagen and antioxidant technology, applied in the field of peptides

Active Publication Date: 2016-01-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few related studies on the preparation and activity of swim bladder protein peptides. Therefore, the preparation of peptides from the swim bladder of farmed large yellow croaker and the study of their antioxidant and anti-fatigue activities provide theoretical and experimental basis for the development of marine drugs and health food

Method used

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  • Swim bladder collagen anti-oxidation and anti-fatigue protein peptide
  • Swim bladder collagen anti-oxidation and anti-fatigue protein peptide
  • Swim bladder collagen anti-oxidation and anti-fatigue protein peptide

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Embodiment

[0013] Example: The swim bladder of cultured large yellow croaker was used as a raw material to prepare polypeptides and their antioxidant and anti-fatigue activities were studied to provide theoretical and experimental basis for the development of marine drugs and health foods. The preparation method of swim bladder collagen multifunctional peptide, it comprises the following steps:

[0014] 1) Cleaning steps: Select swim bladders and wash them thoroughly with running water, cut them into small pieces (0.5×0.5cm). Use 0.1M NaOH at a ratio of 1:10 (w / v), change the solvent every 4 hours, and then wash with cold distilled water until neutral to remove non-toxic substances. Collagen and other miscellaneous proteins; in order to degrease, soak in 10% n-butanol at a ratio of 1:10 (w / v) for 12 hours, change the solvent every 3 hours, and then rinse with 10 times the volume of cold distilled water; soak at 85 % ethanol for 12h, change the solvent every 3h, and finally rinse with c...

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Abstract

The invention relates to swim bladder collagen anti-oxidation and anti-fatigue protein peptide. The amino acid sequence of the swim bladder collagen anti-oxidation and anti-fatigue protein peptide is Gly-Glu-Pro-Gly-Pro-Met-Gly-Leu-Ala-Gly-Pro-Leu-Gly-Pro-Ala-Gly-Gly-Ser-Tyr-Glu-Ser-Lys-Pro-Asn-His, and the molecular weight of the swim bladder collagen anti-oxidation and anti-fatigue protein peptide is 6719.5718Da. The protein peptide has the advantages of being safe, free of toxic and side effects, capable of resisting oxidation and fatigue, high in activity, and the like.

Description

Technical field [0001] The present invention involves a polypeptide extracted from aquatic creatures, especially a type of codonite collagen antioxidant anti -fatigue protein peptide. Background technique [0002] Free radicals are intermediate products of many biochemical reactions of the body. Under normal circumstances, they are in a balanced state.However, when this balance is broken, the free radicals will cause the body to be under the pressure of oxidation. By attacking the macromolecular substances and various cyters of life, the body's various injuries of the molecular, cell and tissue organs, accelerate the body’s body’sAging, fatigue and inducing various diseases. [0003] According to the theory of free radicals, the generation of human fatigue is related to the generating free radical generation, so substances with antioxidant activity have potential anti -fatigue activity. [0004] Natural biological activity substances have a healthy and safe advantage compared t...

Claims

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Application Information

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IPC IPC(8): C07K14/46A61K38/17A61P39/06A61P39/00
CPCA61K38/00C07K14/461
Inventor 丁国芳曾丽徐银峰贾盈露余方苗陈锐
Owner ZHEJIANG OCEAN UNIV
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