Preparation method and application of a molecularly imprinted electrochemiluminescent sensing material

A technology of molecular imprinting and sensing materials, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of complex operation, no research, low sensitivity, etc., and achieve high sensitivity and low detection limit. , good stability

Inactive Publication Date: 2018-05-18
TIANJIN UNIV OF SCI & TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used detection method is chromatography, which is complicated to operate and has low sensitivity.
Molecularly imprinted sensing materials are currently being used for the detection of multiple components, with simple operation and high accuracy, but there is no research on 2-methyl-4-chlorophenoxyacetic acid molecularly imprinted electrochemiluminescence sensing materials.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a molecularly imprinted electrochemiluminescent sensing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of gold nanoparticles functionalized with 2-mercaptoethanesulfonic acid and p-aminothiophenol:

[0021] Mix 10mL of ethanol solution containing 197mg of chloroauric acid with 5mL of methanol solution containing 42mg of sodium mercaptoethanesulfonate and 8mg of p-aminothiophenol, add 2.5mL of glacial acetic acid and incubate in an ice bath for 1 hour; then add 7.5mL of 1mol / L sodium borohydride aqueous solution was prepared into a colloidal gold solution; the above colloidal gold solution was stirred for 1 h under ice bath conditions and then continued to stir for 14 h under normal temperature conditions to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized Modified gold nanoparticle dispersion; the functionalized modified gold nanoparticle dispersion is washed and centrifuged twice with methanol and ethanol respectively, and vacuum-dried to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized of gold nanopartic...

Embodiment 2

[0031] Determination of MCPA content in actual samples:

[0032] Using the working electrode modified by the sensing material of the present invention to analyze and measure MCPA in actual samples (tap water, lake water, river water, oats, wheat), MCPA was not detected, and the standard addition method was used to carry out the standard addition recovery experiment. The electrochemiluminescence and cyclic voltammetry simultaneous detection mode of the LK5100 electrochemiluminescence analysis system was selected for scanning measurement of the sample solution to be tested, the scanning voltage was 0-2.2V, and the photomultiplier tube voltage was 800V. Read the luminescence intensity after electrochemiluminescence quenching, and substitute it into (F 0 -F) / F 0 =0.1006logC MCPA +1.1480, calculated C MCPA . Three parallel measurements were performed, and the calculated recovery rate was 89.2-106.6%, indicating that the electrochemiluminescence sensor constructed by the sensing...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and application of an adsorbed trace amount 2-methyl-4-chlorophenoxyacetic acid (MCPA) molecularly imprinted electrochemical luminescence sensing material based on quantum dot electrochemiluminescence. The preparation method of this sensing material: prepare the functional material of gold nanoparticles and CdSe / ZnS quantum dots of sodium 2-mercaptoethanesulfonate and p-mercaptothiophenol functional modification, and mix MCPA with the prepared functional material , use electrochemical polymerization to form a film to modify the material on the surface of the gold electrode, and prepare the electrode modified by the dianiline cross-linked nano-gold and the quantum dot network array molecularly imprinted electrochemiluminescent material after elution of MCPA. The invention has the advantage of adopting the method of electropolymerization to synthesize the quantum dot molecule imprinted electrochemiluminescence sensing material in one step. The sensing material combines the electrochemiluminescence of quantum dots with the specific recognition of molecularly imprinted polymers, overcomes the shortcomings of existing methods for preparing solid-state light-emitting electrodes, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of preparation of photoelectrochemical sensing materials, and in particular relates to a preparation method of an adsorbed trace amount of 2-methyl-4-chlorophenoxyacetic acid molecularly imprinted electrochemiluminescence sensing material based on quantum dot electrochemiluminescence and application. Background technique [0002] In recent years, with the continuous development of nanotechnology, quantum dots have developed rapidly as a new type of luminescent material. Their excellent luminescent properties have attracted the attention of many scientific researchers. They are widely used in sensing detection, biological imaging, biological probes and other fields. . It has been reported in the literature that quantum dot modified electrodes can be used for the detection of electrochemiluminescence. However, these methods lack specificity and cannot selectively adsorb the target and achieve accurate detect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 方国臻杨钰昆王俊平王硕
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap