A method for rapid restoration of demineralized dentin

A mineral tooth, essential technology, applied in the field of biomaterials, can solve complex and clinically practicable problems, achieve the effect of restoring hierarchical structure, good biocompatibility, and preventing demineralization

Active Publication Date: 2018-03-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such an approach takes up to four months and is not clinically practical
Moreover, the device is composed of slow-release calcium ion sources, phosphorus ion sources, non-collagen analogues, etc., which are too complicated to relieve clinicians from their busy work.

Method used

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  • A method for rapid restoration of demineralized dentin
  • A method for rapid restoration of demineralized dentin
  • A method for rapid restoration of demineralized dentin

Examples

Experimental program
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Effect test

Embodiment 1

[0025]Equipped with 0.25M calcium chloride (CaCl2) aqueous solution, 0.15M disodium hydrogen phosphate (Na2HPO4) aqueous solution, and 10g / L polyacrylic acid (PAA) aqueous solution as mother liquor for synthesis experiments. Mix 2.4 mL of Na2HPO4 mother liquor and 3.30 mL of PAA mother liquor, dilute with double distilled water, adjust the pH to 9.5±0.1 with 1M NaOH aqueous solution, stir at room temperature for 0.5 h, and the total volume is 30 mL. Take 2.4 mL of CaCl2 stock solution and add water to dilute to 30 mL. Add 30 mL of calcium solution 30 to 30 mL of phosphorus solution with a buret, stir with a magnetic stirrer during the dropwise addition, and keep the pH in the range of 9.5±0.5 with 0.1M NaOH solution. Finally, the calcium ion concentration was 10.0 mM, the phosphorus ion concentration was 6.0 mM, and the PAA concentration was 50 ug / mL. Ensure that the entire dropping process lasts for 25 to 30 minutes. Collect caries-free isolated teeth, use a slow-speed ISOM...

Embodiment 2

[0027] Equipped with 0.25 M calcium chloride (CaCl 2 ) aqueous solution, 0.15M disodium hydrogen phosphate (Na 2 HPO 4 ) aqueous solution, 10 g / L polyacrylic acid (PAA) aqueous solution was used as the mother liquor for the synthesis experiment. Mix 2.4 mL of Na2HPO4 mother liquor and 1.2 mL of PAA mother liquor, dilute with double distilled water, adjust the pH to 9.5±0.1 with 1M NaOH aqueous solution, stir at room temperature for 0.5 h, and the total volume is 30 mL. Take 2.4 mL of CaCl 2 Dilute the mother liquor with water to 30 mL. Add 30 mL of calcium solution 30 to 30 mL of phosphorus solution with a buret, stir with a magnetic stirrer during the dropwise addition, and keep the pH in the range of 9.5±0.5 with 0.1M NaOH solution. Finally, the calcium ion concentration was 10.0 mM, the phosphorus ion concentration was 6.0 mM, and the PAA concentration was 200 ug / mL. Ensure that the entire dropping process lasts for 25 to 30 minutes. To collect caries-free isolated t...

Embodiment 3

[0029] Equipped with 0.25M calcium chloride (CaCl 2 ) aqueous solution, 0.15M disodium hydrogen phosphate (Na 2 HPO 4 ) aqueous solution, 10g / L polyacrylic acid (PAA) aqueous solution was used as the mother liquor for the synthesis experiment. Take 2.4 mL Na 2 HPO 4 The mother liquor was mixed with 2.1 mL of PAA mother liquor, diluted with double distilled water, adjusted to pH 9.5±0.1 with 1M NaOH aqueous solution, stirred at room temperature for 0.5 h, and the total volume was 30 mL. Take 2.4 mL of CaCl2 stock solution and add water to dilute to 30 mL. Add 30 mL of calcium solution 30 to 30 mL of phosphorus solution with a burette, stir with a magnetic stirrer during the dropwise addition, and keep the pH in the range of 9.5±0.5 by adjusting the pH with 0.1M NaOH solution. The final concentrations of calcium ions and phosphorus ions were 10.0 mM and 6.0 mM, respectively. Finally, the calcium ion concentration was 10.0 mM, the phosphorus ion concentration was 6.0 mM, a...

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Abstract

The invention relates to a method of quickly repairing demineralized dentine. An existing method of repairing demineralized dentine uses two dentine non-collagen analogs, repairing is implemented by the aid of a Portland cement slow-release system, but this method is too complex and takes as long as four months to achieve full mineralization. According to the method of the invention, polyacrylic acid simple and easy to obtain is used as a regulator, calcium ions and phosphorus ions are used as raw materials, and fluidic amorphous calcium phosphate particles 15-20 nm in size are synthesized; glutamic acid is then used as a mineralization accelerator to mineralize the demineralized dentine again within 2 days, the natural hierarchical structure of the demineralized dentine is repaired, and the mechanical properties of the re-mineralized dentine also close to those of natural dentin. Compared with existing re-mineralization techniques, the method has the advantages that on the basis of achieving equivalent repaired hierarchical structure and mechanical properties, an apparatus is simple, time consumption is low, and the problem of re-mineralizing the demineralized dentine is more effectively solved.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a method for quickly repairing demineralized dentin. Background technique [0002] During the dentin bonding process, the exposed collagen at the base of the mixed layer cannot be completely covered by the adhesive, and the degradation of the exposed collagen by endogenous matrix metalloproteinases is a key factor that shortens the durability of the resin-dentin bonding complex. Moderate and deep carious lesions are also mainly manifested by demineralization of the dentin layer. Addressing these problems involves remineralization of demineralized dentin. With the deepening of research, the microstructure of dentin, the connection between mineralization sites and collagen fibers, the role of non-collagen in dentin biomineralization and the biomineralization process have been gradually revealed by scientists, and biomimetic in vitro Analog, The field of restoration of deminera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K6/033A61K6/838
Inventor 顾新华孙健陈怡陈超群茅彩云王伟朱欣欣王晶王建明
Owner ZHEJIANG UNIV
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