A kind of method for quickly breeding seedlings of Australian tea oil tree aseptic cuttings

Abstract

The invention belongs to the technical field of economic forest tree seedling raising and particularly relates to a method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia. The method includes the following steps of (1) selection and processing of an explant material, (2) establishment of sterile strains for the first time, (3) multiplication culture, (4) rooting culture and (5) transplantation. The method utilizes a cutting technology and also utilizes a tissue culture technology, and therefore the method is high in cutting efficiency and is not affected by the environment. The source problem of cuttage branches is solved through self in-vitro propagation without forest tree consumption, tree growth and essential oil extraction are not influenced, and meanwhile the propagation efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of growing seedlings of economic forests, and in particular relates to a method for rapidly growing seedlings of Australian tea oil tree through aseptic cuttings. Background technique [0002] Australian camellia oil tree, also known as Melaleuca alternifolia, belongs to the family Myrtaceae, and its origin is Australia. my country began to introduce and cultivate it in the 1970s, and it has been promoted and planted in Guangdong, Guangxi, Fujian, Yunnan and other places. Its fresh branches and leaves can extract essential oil, called Melaleuca alternifolia oil, commonly known as tea tree oil (Tea Tree oil). Its main components are eucalyptol and terpine-4-ol, which can efficiently, non-toxically and non-irritatingly kill fungi and bacteria on the surface of human skin, and have inhibitory effect on some viruses. It is a natural fragrance and antibacterial agent And preservatives, so it is widely used in me...

AI Technical Summary

Problems solved by technology

[0008] Disadvantages: It is very expensive to buy imported seeds, about 70 yuan / g; the trees cultivated by oneself need to consume a certain amount of trees, which can no longer be used for the production of essential oils; the seedlings cultivated by seeds have differentiation in their offspring genotypes , can not guarantee the consistent performance of plantation forest growth and essential oil content; the seedling time is affected by the external climate, and the seedlings cannot be produced in factories all year round; the propagation speed is slow and the reproduction coefficient is low

[0012] Disadvantages: The source of scion requires a certain amount of female parent trees; the rooting rate is low, and the germplasm resources are seriously wasted; the reproduction rate is lower than that of tissue culture, the reproduction coefficient is low, and it is greatly affected by the external climate

[0015] Disadvantages are: need facilities, high technical content, high cost

It cannot form a year-round, factory-like, high-reproductive-coefficient seedling-raising technology that does not depend on external climate influences.

At the same time, the introduction of the conventional rapid propagation method of tissue culture (the callus method or the adventitious bud method) requires high technical requirements, and the research and development cycle from theoretical research to real industrial seedling cultivation is long, which cannot be realized in the cultivation of Australian tea oil tree seedlings.

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