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Method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia

A technology for fast-growing seedlings and camellia oil trees, which is applied in the field of economic forest tree seedling raising, can solve the problems of expensive imported seeds, low reproduction rate, large influence, etc., and achieves the effects of high cutting efficiency and improved reproduction efficiency.

Inactive Publication Date: 2016-02-24
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Disadvantages: It is very expensive to buy imported seeds, about 70 yuan / g; the trees cultivated by oneself need to consume a certain amount of trees, which can no longer be used for the production of essential oils; the seedlings cultivated by seeds have differentiation in their offspring genotypes , can not guarantee the consistent performance of plantation forest growth and essential oil content; the seedling time is affected by the external climate, and the seedlings cannot be produced in factories all year round; the propagation speed is slow and the reproduction coefficient is low
[0012] Disadvantages: The source of scion requires a certain amount of female parent trees; the rooting rate is low, and the germplasm resources are seriously wasted; the reproduction rate is lower than that of tissue culture, the reproduction coefficient is low, and it is greatly affected by the external climate
[0015] Disadvantages are: need facilities, high technical content, high cost
It cannot form a year-round, factory-like, high-reproductive-coefficient seedling-raising technology that does not depend on external climate influences.
At the same time, the introduction of the conventional rapid propagation method of tissue culture (the callus method or the adventitious bud method) requires high technical requirements, and the research and development cycle from theoretical research to real industrial seedling cultivation is long, which cannot be realized in the cultivation of Australian tea oil tree seedlings.

Method used

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  • Method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Selection and processing of explant materials:

[0056] When the weather is fine, select young stems from Australian tea oil tree saplings that are free from diseases and insect pests, grow vigorously, and have a diameter of less than 1 mm; cut the young stems into 3 cm lengths, place them under tap water, and stir them While rinsing for 1 hour, first disinfect the surface with 75% alcohol by volume on the ultra-clean workbench for 30 seconds, and then use 0.1% HgCl 2 Disinfect for 6 minutes, rinse 5 times with sterile water for use;

[0057] (2) Establishment of sterile strains for the first time:

[0058] Cut the young and tender stems after the disinfection treatment in step (1) into 2cm long stems, then insert them vertically into the medium according to the growth direction, cut 5 sections in each medium, place them in a room with a temperature of 25°C and an illumination of 2000lx. Cultivate in the environment, 10h light / 14h dark every day; cultivate for 8 w...

Embodiment 2

[0066] (1) Selection and processing of explant materials:

[0067] When the weather is fine, select young stems from Australian tea oil tree saplings that are free from diseases and insect pests, grow vigorously, and have a diameter of less than 1 mm; cut the young stems into 2 cm lengths, place them under tap water, and stir them While washing for 2 hours, first use 75% alcohol to disinfect the surface for 60 seconds on the ultra-clean workbench, and then use 0.1% HgCl 2 Disinfect for 7 minutes, rinse with sterile water 4 times for use;

[0068] (2) Establishment of sterile strains for the first time:

[0069] Cut the young stems after disinfection in step (1) into 1 cm long stems, then insert them vertically into the culture medium according to the growth direction, cut 5 sections in each culture medium, place them in a room with a temperature of 25 °C and an illumination of 1500 lx. Culture in the environment, 10h light / 14h dark every day; culture for 13 weeks;

[0070] ...

Embodiment 3

[0077] (1) Selection and processing of explant materials:

[0078] When the weather is fine, select young stems from Australian tea oil tree saplings that are free from diseases and insect pests, grow vigorously, and have a diameter of less than 1mm; cut the young stems into 2.5cm lengths, place them under tap water, and Rinse while stirring for 1.5h, first use alcohol with a volume fraction of 75% to carry out surface disinfection for 40s on the ultra-clean workbench, and then use 0.1% HgCl 2 Disinfect for 6.5 minutes, rinse with sterile water 7 times for use;

[0079] (2) Establishment of sterile strains for the first time:

[0080] Cut the young stems after disinfection in step (1) into 1.5cm long stems, then insert them vertically into the medium according to the growth direction, cut 5 sections in each medium, place them at a temperature of 25°C, and an illumination of 1800lx Cultivated in an environment with 10h light / 14h darkness every day; cultured for 10 weeks;

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Abstract

The invention belongs to the technical field of economic forest tree seedling raising and particularly relates to a method for sterile cottage and rapid seedling propagation and raising of Australian melaleuca alternifolia. The method includes the following steps of (1) selection and processing of an explant material, (2) establishment of sterile strains for the first time, (3) multiplication culture, (4) rooting culture and (5) transplantation. The method utilizes a cutting technology and also utilizes a tissue culture technology, and therefore the method is high in cutting efficiency and is not affected by the environment. The source problem of cuttage branches is solved through self in-vitro propagation without forest tree consumption, tree growth and essential oil extraction are not influenced, and meanwhile the propagation efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of growing seedlings of economic forests, and in particular relates to a method for rapidly growing seedlings of Australian tea oil tree through aseptic cuttings. Background technique [0002] Australian camellia oil tree, also known as Melaleuca alternifolia, belongs to the family Myrtaceae, and its origin is Australia. my country began to introduce and cultivate it in the 1970s, and it has been promoted and planted in Guangdong, Guangxi, Fujian, Yunnan and other places. Its fresh branches and leaves can extract essential oil, called Melaleuca alternifolia oil, commonly known as tea tree oil (TeaTree oil). Its main components are eucalyptol and terpine-4-ol, which can efficiently, non-toxically and non-irritatingly kill fungi and bacteria on the surface of human skin, and have inhibitory effect on some viruses. It is a natural fragrance and antibacterial agent And preservatives, so it is widely used in med...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 郭雁君吉前华黄甫民
Owner ZHAOQING UNIV
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