A cellulosic alga rich in oil and its cultivation application
A fiber and oil technology, applied in the field of cellulosic algae and their cultivation applications, can solve the problems of not involving oil content, affecting the growth of algae cells, and not using them, so as to solve the problem of oil source, avoid photosynthesis inhibition, and fast growth rate. Effect
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Embodiment 1
[0021] Example 1 Separation and acclimatization screening to obtain Monostipella SS-B7
[0022] (1) Obtaining the starting algae strain: In October 2011, 50 mL of water samples were retrieved from Longhu Lake in Shoushan Leisure Resort, Dubolte Mongolian Autonomous County, Heilongjiang, and inoculated into 200 mL of BG11 medium for enrichment culture. The light intensity for cultivation is 5000 Lux, the temperature is 25°C, the light-dark cycle is 24h, and the light-dark time ratio is 14:10. After about half a month of cultivation, the culture medium turns green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000 Lux, and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultivation, the cultivation temperature is 25°C, and the li...
Embodiment 2
[0027] Example 2 Identification of algae strains
[0028] The DNA of the obtained SS-B7 algae cells was extracted by the CTAB method, and the 18S rDNA gene was cloned, and the three positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Ankistrodesmus sp. It has the greatest similarity, its BLASTn value is 2702, and its Max index value is 99%. It can be determined that SS-B7 is a cellulosic algae ( Ankistrodesmus sp. ).
Embodiment 3
[0029] The cultivation application of embodiment 3 cellulosus SS-B7
[0030] The algae liquid of SS-B7 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formulation of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a photobioreactor. The OD of the culture solution after inoculation was 690 is 0.2. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 30v%, of which NO 2 The content is 300×10 -6 (v / v). During the cultivation process, the light intensity was 5000Lux, the cultivation temperature was 28°C, the pH value was controlled between 7-9, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured to calculate the biomass production, and th...
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