BET inhibition therapy for heart disease
A therapy, cardiomyopathy technology, applied in one area of that can address high morbidity and mortality
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[0206] Animal Models. All protocols for animal use were approved by the Institutional Animal Care and Use Committee at Case Western Reserve University and performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals. All models were performed in C57B1 / 6J mice (Jackson Laboratories) maintained in a pathogen-free facility under standard light / dark cycles with ad libitum access to food and water.
[0207] Human samples. LV samples from healthy human hearts were as described (Harley et al., 2006; Margulies et al., 2005) according to the Investigation Review Committee at the Hospital of the University of Pennsylvania, Philadelphia, PA. , Philadelphia, PA). Nucleoproteins were extracted using the NE-Per kit (Thermo Scientific #78833) according to the manufacturer's instructions. Gene expression profiles of the left ventricle obtained from non-failing and failing human hearts were curated from a publicly available dataset (Hainen-Harry et a...
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