A compound microecological preparation for preventing and controlling poultry enteropathogens and preparation method thereof

A composite micro-ecology, chick technology, applied in the field of applied microorganisms, can solve the problems of not being able to truly meet the requirements of replacing antibiotics, inhibiting intestinal pathogens without pertinence, etc., to promote the development of chick immune system, strong antibacterial activity, Significant effect of pathogen prevention and control

Active Publication Date: 2019-03-08
FUJIAN BRADY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its action requires a process, especially in the inhibition of intestinal pathogens, it is not targeted, and it cannot really meet the requirements of replacing antibiotics

Method used

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  • A compound microecological preparation for preventing and controlling poultry enteropathogens and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Effects of Bacillus polymyxa BPZ-1 and its culture medium on Salmonella and Escherichia coli

[0032] Prepare beef extract peptone medium and its solid medium for use. The components of beef extract peptone medium are as follows: each liter of medium contains beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, pH 7.2-7.4 , sterilized at 121°C for 30min, and added 1.5% agar powder to the solid medium. Use beef extract peptone slant to activate Paenibacillus polymyxa BPZ-1, CGMCC preservation number: 11255, and then inoculate in 100 mL of beef extract peptone medium, culture at 30° C. for 24 hours before use. The activated Salmonella and Escherichia coli were mixed with sterile water and then coated on the beef extract peptone solid plate, and 3 sterile Oxford cups were evenly placed into the petri dish with tweezers, and 0.2ml of beef extract peptone was respectively drawn. The culture medium, the Bacillus polymyxa BPZ-1 culture medium and the Bacillus po...

Embodiment 2

[0033] Example 2: Effects of Lactobacillus casei LCZ-1 and its culture medium on Salmonella and Escherichia coli

[0034] Prepare MRS agar and MRS broth medium for use. The components of MRS agar medium are as follows: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 80ml, K 2 HPO 4 2g, Sodium Acetate 5g, Diammonium Citrate 2g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O 0.05g, agar powder 15g, add distilled water to 1L, sterilize with an autoclave at 121°C for 15min, and adjust the pH value to 6.2-6.4. The composition of MRS broth medium is MRS agar medium and the agar powder is removed. Lactobacillus casei LCZ-1 was activated with MRS slant, CGMCC deposit number: 11256, and then inoculated into 100 ml of MRS broth medium, cultured at 30° C. for 24 hours before use. Prepare beef extract peptone solid medium: beef extract 5g, peptone 10g, NaCl 5g, add water to 1L, adjust pH to 7.2-7.4, sterilize at 121°C for 20min in an autoclave, pour the solid plate. The a...

Embodiment 3

[0036] Example 3: Preparation of Bacillus polymyxa inoculum

[0037] (1) Configuration of culture medium at all levels, including:

[0038] Slant culture medium: Beef extract peptone solid medium, for use;

[0039] Shake flask bacterial culture medium and seed tank fermentation medium: Beef extract peptone liquid medium, standby;

[0040] Mass production medium: corncob flour 45%, bran 40%, husk bran: 10%, glucose 3%, yeast extract 2%, add water according to the material-water ratio of 1:0.8;

[0041] (2) Shake-flask strain expansion: Bacillus polymyxa BPZ-1 activated with slant medium was placed in 100 mL of shake-flask medium under aseptic operation, and shaken at 30°C at 160 r / min. In the bed, after culturing for 24h, it is ready for use;

[0042] (3) seed tank fermentation: put the step (1) seed tank fermentation medium into the seed tank, carry out steam high temperature sterilization and cooling in the seed tank, insert the shake flask of step (2) according to the ino...

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Abstract

The invention discloses a compound microbial ecological preparation for preventing and controlling enteric pathogens of young poultry and a preparation method of the preparation, and relates to the technical field of applied microbiology. The compound microbial ecological preparation comprises bacillus polymyxa BPZ-1, CGMCC No.11255 and lactobacillus casei LCZ-1, CGMCC No.11256, and the bacillus polymyxa BPZ-1, CGMCC No.11255 and the lactobacillus casei LCZ-1, CGMCC No.11256 are subjected to solid state fermentation and compounding in proportion to obtain the compound microbial ecological preparation. When the compound microbial ecological preparation is used, the young poultry can take the compound microbial ecological preparation through water when the young poultry open mouths, and then the compound microbial ecological preparation is added to feed for feeding for a week. The compound microbial ecological preparation has strong effects on inhibiting escherichia coli and salmonella, diseases such as diarrhea of the young poultry are effectively protected and cured, growth of intestinal flora of the young poultry is promoted, the survival rate of the young poultry is increased, and the growth performance is improved. The microbial preparation is a good replacing antibiotic and is a special novel microbial ecological preparation which prevents and controls the enteric pathogens of the young poultry, protects and cures the diarrhea of the young poultry and promotes normal development of the intestinal flora of the young poultry; preparation is simple, the significant application effect is achieved, and the compound microbial ecological preparation is one of important ways of achieving green poultry breeding.

Description

technical field [0001] The invention relates to the technical field of applied microorganisms, in particular to a composite microbial preparation for preventing and controlling intestinal pathogens of chicks and a preparation method thereof. Background technique [0002] In the current process of large-scale and intensive development of poultry breeding, the healthy breeding of chicks plays a key role in the success or failure of breeding. At the stage from hatching from the breeder farm to entering the farm, the chicks have weak physique, incomplete development of intestinal flora, and poor resistance. Inflammation and other diseases lead to high mortality and seriously affect the breeding efficiency. In current breeding practice, antibiotics are often used to prevent or treat diseases that chicks are prone to produce, and they are administered as soon as the chicks eat. However, the abuse of antibiotics in chicks inhibits the establishment of normal intestinal flora, aff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/747A61P1/00A61P31/04C12N1/20C12R1/12C12R1/245A61K35/742
Inventor 赵军肖建设杨荣平潘慧青张炎达赵清国史金慧邓翠芬林贺桂王新
Owner FUJIAN BRADY PHARMA CO LTD
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