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Compound microbial ecological preparation for preventing and controlling enteric pathogens of young poultry and preparation method of preparation

A compound micro-ecology and preparation technology, applied in the field of applied microorganisms, can solve the problems of untargeted inhibition of intestinal pathogens and the inability to truly meet the requirements of replacing antibiotics, achieving strong bacteriostatic activity, promoting the development of the immune system of chicks, and targeting at strong effect

Active Publication Date: 2016-03-02
FUJIAN BRADY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its action requires a process, especially in the inhibition of intestinal pathogens, it is not targeted, and it cannot really meet the requirements of replacing antibiotics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Bacillus polymyxa BPZ-1 and its culture fluid are to the effect of Salmonella and Escherichia coli

[0032] Prepare beef extract peptone medium and its solid medium for future use. The ingredients of beef extract peptone medium are as follows: Each liter of medium contains 5.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, and a pH value of 7.2-7.4 , sterilized at 121°C for 30 minutes, and added 1.5% agar powder to the solid medium. Activation of Bacillus polymyxa with beef extract peptone slant ( Paenibacillus polymyxa ) BPZ-1, CGMCC preservation number: 11255, then inoculated in 100 mL of beef extract peptone medium, cultured at 30° C. for 24 hours, and then used for later use. Mix the activated Salmonella and Escherichia coli with sterile water and spread them on the beef extract peptone solid plate, put 3 sterile Oxford cups into the petri dish evenly with tweezers, and absorb 0.2ml of beef extract peptone respectively Culture medium, B...

Embodiment 2

[0033] Embodiment 2: Lactobacillus casei LCZ-1 and its culture fluid are to the effect of Salmonella and escherichia coli

[0034] Prepare MRS agar and MRS broth medium, spare, the composition of MRS agar medium is as follows: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 80ml, K 2 HPO 4 2g, sodium acetate 5g, diammonium citrate 2g, MgSO 4 7H 2 O0.2g, MnSO 4 4H 2 O0.05g, 15g of agar powder, add distilled water to 1L, sterilize at 121°C for 15min with an autoclave, and adjust the pH value to 6.2-6.4. The composition of MRS broth medium is MRS agar medium minus agar powder. Activation of Lactobacillus casei with MRS slant ( Lactobacillus casei ) LCZ-1, CGMCC preservation number: 11256, then inoculated in 100 ml of MRS broth medium, cultured at 30°C for 24 hours and then used for later use. Prepare beef extract-peptone solid medium: 5g beef extract, 10g peptone, 5g NaCl, add water to 1L, adjust pH to 7.2-7.4, sterilize at 121°C for 20min in an autocl...

Embodiment 3

[0036] Embodiment 3: the preparation of bacillus polymyxa bacterial agent

[0037] (1) Configuration of media at all levels, including:

[0038] Incline medium: beef extract peptone solid medium, spare;

[0039] Shake flask culture medium and seed tank fermentation medium: beef extract peptone liquid medium, spare;

[0040] Mass production medium: 45% corn cob powder, 40% bran, 10% rice bran, 3% glucose, 2% yeast extract, adding water according to the ratio of material to water: 1:0.8;

[0041] (2) Shake flask strain expansion: put the Bacillus polymyxa BPZ-1 activated with slant medium into 100mL shake flask medium under aseptic operation, and shake at 30°C and 160rpm / min In the bed, after culturing for 24 hours, it is ready for use;

[0042] (3) Seed tank fermentation: put the seed tank fermentation medium in step (1) into the seed tank, perform steam high-temperature sterilization in the seed tank and cool it down, and then insert 8% of the inoculum into the shake flask ...

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Abstract

The invention discloses a compound microbial ecological preparation for preventing and controlling enteric pathogens of young poultry and a preparation method of the preparation, and relates to the technical field of applied microbiology. The compound microbial ecological preparation comprises bacillus polymyxa BPZ-1, CGMCC No.11255 and lactobacillus casei LCZ-1, CGMCC No.11256, and the bacillus polymyxa BPZ-1, CGMCC No.11255 and the lactobacillus casei LCZ-1, CGMCC No.11256 are subjected to solid state fermentation and compounding in proportion to obtain the compound microbial ecological preparation. When the compound microbial ecological preparation is used, the young poultry can take the compound microbial ecological preparation through water when the young poultry open mouths, and then the compound microbial ecological preparation is added to feed for feeding for a week. The compound microbial ecological preparation has strong effects on inhibiting escherichia coli and salmonella, diseases such as diarrhea of the young poultry are effectively protected and cured, growth of intestinal flora of the young poultry is promoted, the survival rate of the young poultry is increased, and the growth performance is improved. The microbial preparation is a good replacing antibiotic and is a special novel microbial ecological preparation which prevents and controls the enteric pathogens of the young poultry, protects and cures the diarrhea of the young poultry and promotes normal development of the intestinal flora of the young poultry; preparation is simple, the significant application effect is achieved, and the compound microbial ecological preparation is one of important ways of achieving green poultry breeding.

Description

technical field [0001] The invention relates to the technical field of applied microorganisms, in particular to a compound micro-ecological preparation for preventing and controlling intestinal pathogens of poultry and a preparation method thereof. Background technique [0002] In the process of large-scale and intensive development of poultry farming, the healthy breeding of young poultry plays a key role in the success or failure of farming. However, at the stage of hatching from the breeder farm to entering the farm, the chicks have weak constitution, imperfect development of intestinal flora, poor resistance, and are very susceptible to diarrhea, umbilical Inflammation and other diseases lead to higher mortality and seriously affect the breeding efficiency. In current farming practices, antibiotics are mostly used to prevent or treat diseases that chicks are prone to, and the administration starts when the chicks start eating. However, the abuse of antibiotics in young...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/00A61P31/04C12N1/20C12R1/12C12R1/245A61K35/742
Inventor 赵军肖建设杨荣平潘慧青张炎达赵清国史金慧邓翠芬林贺桂王新
Owner FUJIAN BRADY PHARMA CO LTD
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