Culture medium additive for inducing taxol cells to produce 10-deacetylation baccatin III

Abstract

The invention provides a culture medium additive for inducing taxol cells to produce 10-deacetylation baccatin III. The additive is prepared from sodium molybdate, colchicine, histidine, apigenin, peptone, yeast powder, fructus forsythiae, abrus cantoniensis hance, hyacinth bean coats, aizoon stonecrop herb and the like. The autophage mode of taxol cells is interfered with through the mode of adding inductors to a culture medium, and therefore taxol cells can be induced to secrete the docetaxel semisynthesis precursor 10-deacetylation baccatin III in a directed mode. It is found through experiments that after the additive is added to cell culture fluid, the 10-deacetylation baccatin III secretion amount of taxol cells is remarkably increased; meanwhile, the cell culture density can be increased to a certain degree. The culture medium additive is clear in thought, the technical scheme is reasonable, and good large-scale application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a medium additive for inducing yew cells to produce 10-deacetylbaccatin III. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Docetaxel contains a taxane ring structur...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a medium additive for inducing yew cells to produce 10-deacetylbaccatin III, so as to solve the problems of yew cells to 10-deacetylbaccatin in the prior art. Technical problem with low secretion of mycin III