Culture medium additive for inducing taxol cells to produce 10-deacetylation baccatin III

A medium additive, baccatin technology, applied in the field of cell engineering, can solve the problem of low secretion of 10-deacetylbaccatin III, achieve good prospects for large-scale application, promote production rate, and have reasonable technical solutions Effect

Inactive Publication Date: 2016-03-02
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a medium additive for inducing yew cells to produce 10-deacetylbaccatin III, so as to solve the problems of yew cells to 10-deacetylbaccatin in the prior art. Technical problem with low secretion of mycin III

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A medium additive for inducing yew cells to produce 10-deacetylbaccatin III, consisting of the following ingredients in parts by weight: 8 parts of sodium molybdate, 4 parts of colchicine, 2 parts of histidine, and 1 part of apigenin 1 part of peptone, 1 part of yeast powder, 1 part of forsythia, 1 part of chicken bone grass, 1 part of lentil coat, 1 part of sedum notoginseng, and 50 parts of water.

Embodiment 2

[0019] A medium additive for inducing yew cells to produce 10-deacetylbaccatin III, consisting of the following components by weight: 10 parts of sodium molybdate, 6 parts of colchicine, 3 parts of histidine, and 2 parts of apigenin 2 parts of peptone, 2 parts of yeast powder, 2 parts of forsythia, 2 parts of chicken bone grass, 2 parts of lentil coat, 2 parts of sedum notoginseng, 60 parts of water.

Embodiment 3

[0021] A medium additive for inducing yew cells to produce 10-deacetylbaccatin III, consisting of the following ingredients in parts by weight: 9 parts of sodium molybdate, 5 parts of colchicine, 2.3 parts of histidine, and 1.8 parts of apigenin 1.5 parts of peptone, 1.6 parts of yeast powder, 1.7 parts of forsythia, 1.8 parts of chicken bone grass, 1.2 parts of lentils, 1.8 parts of sedum notoginseng, and 55 parts of water.

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PUM

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Abstract

The invention provides a culture medium additive for inducing taxol cells to produce 10-deacetylation baccatin III. The additive is prepared from sodium molybdate, colchicine, histidine, apigenin, peptone, yeast powder, fructus forsythiae, abrus cantoniensis hance, hyacinth bean coats, aizoon stonecrop herb and the like. The autophage mode of taxol cells is interfered with through the mode of adding inductors to a culture medium, and therefore taxol cells can be induced to secrete the docetaxel semisynthesis precursor 10-deacetylation baccatin III in a directed mode. It is found through experiments that after the additive is added to cell culture fluid, the 10-deacetylation baccatin III secretion amount of taxol cells is remarkably increased; meanwhile, the cell culture density can be increased to a certain degree. The culture medium additive is clear in thought, the technical scheme is reasonable, and good large-scale application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a medium additive for inducing yew cells to produce 10-deacetylbaccatin III. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Docetaxel contains a taxane ring structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12N5/04
CPCC12P17/02C12N5/04C12N2500/05C12N2500/32C12N2500/76C12N2501/999
Inventor 夏雪城夏爽
Owner 天津市博爱生物药业有限公司
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