A kind of antibacterial material and its preparation and application
An antibacterial material and antibody molecule technology, which is applied in the field of antibacterial material preparation and achieves the effects of simple preparation method, low price and excellent biocompatibility
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Embodiment 1
[0047] 1. Preparation of hydroxyapatite:
[0048] At room temperature, 1.791 g of Na 2 HPO 4 12H 2 O was dissolved in 250 ml of deionized water to form solution A, and 1.762 g of Ca(CH 3 COO) 2 ·H 2 O was dissolved in 100 mL of deionized water to form solution B. Add 0.030 g of protidine DNA to 30 ml of solution A, stir at room temperature to dissolve it completely, adjust the pH to 5 with 1 mol / L of hydrochloric acid, then add 10 ml of solution B dropwise, and use 1 mol / L of liters of hydrochloric acid to adjust the pH and keep it at around pH 5. After the dropwise addition was complete, the mixed clear liquid was transferred into a Teflon liner (liner capacity 100 ml) and sealed. Put the lining into the hydrothermal reaction jacket and put the jacket together with the microwave reaction oven to react at 120° C. for 10 minutes. After the reaction system was naturally cooled to room temperature, the product was taken out, and the product was separated by centrifugation...
Embodiment 2
[0056] 1. Preparation of hydroxyapatite:
[0057] At room temperature, 1.791 g of Na 2 HPO 4 12H 2 O was dissolved in 250 ml of deionized water to form solution A, and 1.762 g of Ca(CH 3 COO) 2 ·H 2 O was dissolved in 100 mL of deionized water to form solution B. Add 0.030 g of protidine DNA to 30 ml of solution A, stir at room temperature to dissolve it completely, add 10 ml of solution B, and adjust the pH to 10 with 1 mol / L of sodium hydroxide. Transfer the mixed clear liquid into a Teflon liner (liner capacity 100 ml) and seal. Put the lining into the hydrothermal reaction jacket and put the jacket together with the microwave reaction oven to react at 120° C. for 10 minutes. After the reaction system was naturally cooled to room temperature, the product was taken out, and the product was separated by centrifugation. The separated product was washed three times with deionized water, once with absolute ethanol, and dried in the air at 60°C to obtain the product shown ...
Embodiment 3
[0066] Antibacterial effect experiment
[0067] 1. Experimental materials
[0068] Three common pathogenic bacteria in oral caries and periodontal disease: Streptococcus mutans (UA159), Lactobacillus acidophilus (L.acidophilus, ATCC 4356) and Fusobacterium nucleatum (Fusobacterium nucleatum, ATCC 25586) were selected as samples Bacterial strains for antibacterial experiments.
[0069] 2. Experimental method
[0070] Streptococcus mutans and Fusobacterium nucleatum were resuscitated and cultured in bovine brain infusion (Brain Heart Infusion, BHI), and Lactobacillus acidophilus was cultured in MRS medium. After the bacteria were recovered, they were cultured in an anaerobic box on a solid medium for 48 hours, and independent colonies were picked and placed in a liquid medium, and cultured anaerobically for 24 hours. Take the bacterial suspension in the logarithmic growth phase of the third generation as the experimental bacterial liquid.
[0071] First select the ones prepa...
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