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A method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes

A technology of microcontact printing and giant phospholipids, which is applied in the preparation of test samples, etc., can solve the problem of single size control of giant phospholipid vesicles, and achieve good monodispersity of vesicles, single and controllable size, and wide distribution range Effect

Inactive Publication Date: 2018-07-06
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the giant phospholipid vesicles prepared by the existing method cannot be controlled in a single size, and to provide a method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes

Method used

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  • A method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes
  • A method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes
  • A method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes

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specific Embodiment approach 1

[0025] Embodiment 1: This embodiment is a method for preparing giant phospholipid vesicle arrays based on microcontact printing technology using the electric field of point-surface electrodes, which is completed in the following steps:

[0026] 1. Clean the electrode: first use absolute ethanol to ultrasonically clean the ITO electrode, then use distilled water to ultrasonically clean the ITO electrode, then use nitrogen to dry it, and then use a plasma cleaner to clean the ITO electrode; the cleaned ITO electrode is obtained Electrode: Soak the tungsten needle electrode in hydrochloric acid with a substance concentration of 2mol / L for 3h to 6h, then rinse it with distilled water for 3 to 6 times, and then dry it with nitrogen to obtain the cleaned tungsten needle electrode;

[0027] 2. Cleaning of polydimethylsiloxane stamps with patterns: First, use distilled water to ultrasonically clean the polydimethylsiloxane stamps with patterns, and then use absolute ethanol to clean th...

specific Embodiment approach 2

[0043] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the number of times for ultrasonic cleaning of the ITO electrode described in step 1 using absolute ethanol is 2 to 3 times, and the time for each ultrasonic cleaning is 5min~20min, the ultrasonic power is 40W~80W. Others are the same as the first embodiment.

specific Embodiment approach 3

[0044] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the number of times of using distilled water to ultrasonically clean the ITO electrode described in step one is 2 to 3 times, and the time for each ultrasonic cleaning is 5min~20min, the ultrasonic power is 40W~80W. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a method for preparing a giant phospholipid vesicle array through a point-surface electrode field on the basis of the contact print technology, and relates to a method for preparing the giant phospholipid vesicle array. The method aims at solving the problem that the single size of giant phospholipid vesicle prepared through an existing method can not be controlled. The method includes the steps of firstly, washing an electrode; secondly, washing a polydimethylsiloxane seal with a pattern; thirdly, preparing a phospholipid thin film array; fourthly, preparing an ITO electrode of the patterned phospholipid thin film array; fifthly, assembling a closed preparation device; sixthly, forming the giant phospholipid vesicle array, and obtaining the giant phospholipid vesicle array. The prepared giant phospholipid vesicle array can be widely applied to research and application of the aspects such as high-throughput phospholipid membrane curvature, cell fusion, substance transmission and monomolecular reactions. The method for preparing the giant phospholipid vesicle array through the point-surface electrode field on the basis of the contact print technology can be achieved.

Description

technical field [0001] The invention relates to a method for preparing a giant phospholipid vesicle array. Background technique [0002] The methods for preparing giant phospholipid vesicles mainly include ethanol injection method, calcium fusion method, freeze-drying method, extrusion method, ultrasonic dispersion method, electroforming method, etc., but the size distribution range of giant phospholipid vesicles prepared by these methods is relatively wide, single Size is difficult to control. At present, the preparation of giant phospholipid vesicles with a single and controllable size is rarely reported, and there is no report on the preparation of giant phospholipid vesicle arrays using point-surface electrodes. Contents of the invention [0003] The purpose of the present invention is to solve the problem that the giant phospholipid vesicles prepared by the existing method cannot be controlled in a single size, and provide a method for preparing giant phospholipid ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 韩晓军朱春桃
Owner HARBIN INST OF TECH
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