Method for establishing mint leaf high-efficiency regeneration system

A technology for efficient regeneration of mint leaves, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of short stem tip propagation time and inapplicability of large-scale multiplication of mint varieties, and achieve short regeneration cycle, good repeatability

Inactive Publication Date: 2016-03-16
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

At present, the rapid propagation system for domestic mint varieties mainly uses shoot tips as regeneration materials, but this method i

Method used

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  • Method for establishing mint leaf high-efficiency regeneration system

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Embodiment 1

[0050] The regeneration of adventitious buds from the leaves of mint variety '738' is carried out in several steps in the following order.

[0051] 1. Collection of explants and establishment of sterile system

[0052] Collect mint terminal buds from March to June, soak in washing powder solution for 15 minutes, and rinse with running water for 30 minutes. Before inoculation, disinfect with 75% (V / V) alcohol for 30 seconds, soak in 0.2% (m / V) HgCl2 solution for 15 minutes, and rinse with sterile water three times. After absorbing the surface moisture, the outer leaves were peeled off, cut into 0.5cm long shoot tips, and inoculated in 1.0mg·L -1 In the MS medium of BA and 0.2mg / L NAA, the medium contains 5.6g / L agar, 30g / L sucrose, pH5.5~5.8, and sterilized at 121°C for 20min. The culture temperature is 25°C, the light time is 8~10h, and the light intensity is 1500~2000lx. After three weeks of cultivation, healthy and sterile plants were selected and inoculated in the same m...

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Abstract

The invention relates to a method for establishing a mint leaf high-efficiency regeneration system. The method comprises taking mint aseptic seedling morphology second, third and forth leaves as materials, cutting into small blocks, inducing to an adventitious bud induction medium, researching key technologies such as induction, stretching growth, subculture multiplication, seedling strengthening, rooting and the like, and finally realizing internode stem regeneration. The method comprises the advantages that the induction adventitious bud regeneration rate reaches 95%, stability is good, operation is simple, regeneration period is short, and the established system can be used as an acceptor system for agrobacterium genetic transformation.

Description

technical field [0001] The invention relates to the establishment of a method for efficiently regenerating leaves of mint, a traditional Chinese medicine. Background technique [0002] Mint( Menthahap localyx Briq.) is rich in volatile oil in stems and leaves, and is widely used in food, medicine, cosmetics, spices, tobacco and other industries. The whole herb of mint is used as medicine to treat wind-heat cold, the beginning of wind and temperature, headache, red eyes, sore throat, sore throat, sore tongue, toothache, urticaria, rubella, etc. Mint is one of the important spices and medicinal plants in China. It is widely cultivated in Jiangsu, Anhui, Jiangxi and other places, and has become an important agricultural special economic crop in the local area. However, the phenomenon of single species and species degeneration in mint production has made mint breeding work more and more attractive. The use of transgenic technology to cultivate fine new varieties of mint has t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 于盱梁呈元亓希武房海灵李维林
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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