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Extraction method and application of plant composition containing dendrobium

An extraction method and composition technology, which is applied in the direction of drug combinations, medical preparations containing active ingredients, medical raw materials derived from ferns/filamentous plants, etc., can solve the problems of large dosage for patients, many impurities, and rough processes , to achieve the effect of reducing the dosage

Inactive Publication Date: 2016-03-16
苏州枫灵医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the use of microwave technology in the extraction and preparation of plant compositions containing Dendrobium, but the method of decocting in water is rough, backward, and has many impurities, which leads to excessive dosage for patients, which is inconvenient to take and serious. Affected the clinical application of this product

Method used

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  • Extraction method and application of plant composition containing dendrobium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Get 100 parts of dendrobium, 80 parts of rock chinensis, 50 parts of scutellaria chinensis, 70 parts of bean leaf seven, and 60 parts of Cuiyun grass, grind them, add 2L of 70% ethanol, and put them into a microwave extraction device for microwave extraction. The extraction power is 400-600W, extract twice, 4-8 minutes each time, combine the extracts, concentrate, add to D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, and recover under reduced pressure Ethanol, concentrated and dried to obtain a microwave extract, and prepared into tablets with conventional excipients, each tablet 0.4g.

Embodiment 2

[0016] Get 100 parts of dendrobium, 80 parts of rock chinensis, 50 parts of scutellaria chinensis, 70 parts of bean leaf seven, and 60 parts of Cuiyun grass, grind them, add 2L of 70% ethanol, and put them into a microwave extraction device for microwave extraction. The extraction power is 400-600W, extract twice, 4-8 minutes each time, combine the extracts, concentrate, add to D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, and recover under reduced pressure Ethanol, concentrated and dried to obtain a microwave extract, which is prepared into capsules with conventional auxiliary materials.

Embodiment 3

[0018] Get 100 parts of dendrobium, 80 parts of rock chinensis, 50 parts of scutellaria chinensis, 70 parts of bean leaf seven, and 60 parts of Cuiyun grass, grind them, add 2L of 70% ethanol, and put them into a microwave extraction device for microwave extraction. The extraction power is 400-600W, extract twice, 4-8 minutes each time, combine the extracts, concentrate, add to D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, and recover under reduced pressure Ethanol, concentrated and dried to obtain a microwave extract, which is prepared into granules by adding conventional auxiliary materials.

[0019] Rock tendon is the whole herb of the rock plum family plant white slave flower. The Latin name is Berneuxiathibetica Decne. Shortiathibetica (Decne.) Frachn. Dispelling cold, relieving asthma. For wind-cold cold, cough, asthma, bruises.

[0020] Achyranthes saspera, Latin name: Achyranthesaspera L., is the whole pla...

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Abstract

The invention provides an extraction method of a plant composition containing dendrobium. The plant composition is prepared from 100 parts of dendrobium, 80 parts of tibetan berneuxia, 50 parts of common achyranthes herb, 70 parts of rhodiola yunnanensis and 60 parts of uncinata spikemoss herb. The plant composition is prepared with the microwave extraction method, content is increased greatly, and dosage is reduced. The invention further provides the application of the plant composition containing dendrobium in preparing drugs for inhibiting proliferation of human cervix squamous SiHa cell carcinoma cells.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to an extraction method and application of a plant composition containing dendrobium. Background technique [0002] The botanical composition containing Dendrobium is made from 100 parts of Dendrobium, 80 parts of Rockweed, 50 parts of Daokoucao, 70 parts of Douyeqi, and 60 parts of Cuiyuncao as raw materials. The current extraction method is to add water and decoct twice , every 2-3 hours, combined decoction, filtered, concentrated into a thick paste, granulated, dried, sugar-coated, that is. [0003] In the prior art, there is no report on the use of microwave technology in the extraction and preparation of plant compositions containing Dendrobium, but the method of decocting in water is rough, backward, and has many impurities, which leads to excessive dosage for patients, which is inconvenient to take and serious. Affected the clinical applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P35/00
CPCA61K36/8984A61K36/11A61K36/185A61K36/21A61K36/41A61K2236/333A61K2236/39A61K2236/55A61K2300/00
Inventor 王菊明
Owner 苏州枫灵医药科技有限公司
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