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Fibroblast culture system

A fibroblast and culture system technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problem of low proliferation rate of fibroblasts, and achieve the effect of ensuring purity and promoting growth

Inactive Publication Date: 2016-03-16
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Today's fibroblast culture systems have been obtained in serum-containing media (usually 5-20% serum), but the rate of fibroblast proliferation is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of culture system that promotes the growth of fibroblasts of the present embodiment, its concrete composition is as follows:

[0025] DMEM basal medium

[0026] Fetal Bovine Serum 10%

[0027] aFGF50ng / ml

[0028] Among them, aFGF effectively promotes the growth of fibroblasts and maintains the phenotype of fibroblasts.

Embodiment 2

[0030] A kind of culture system that promotes the growth of fibroblasts of the present embodiment, its concrete composition is as follows:

[0031] DMEM basal medium

[0032] Fetal bovine serum 20%

[0033] aFGF500ng / ml

Embodiment 3

[0035] A kind of culture system that promotes the growth of fibroblasts of the present embodiment, its concrete composition is as follows:

[0036] DMEM basal medium

[0037] Fetal Bovine Serum 15%

[0038] aFGF300ng / ml

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Abstract

The invention discloses a fibroblast culture system, which comprises: a DMEM basic medium, 10%-20% fetal calf serum, and 50-500ng / ml aFGF (Acidic fibroblast growth factor). According to the invention, a new culture system is established, i.e. the aFGF is added into a common culture system to inhibit the fibroblast growth, also proliferation of stem cells is not affected, and the cell purity is ultimately ensured.

Description

technical field [0001] The invention relates to a fibroblast culture system, which belongs to the field of stem cell culture. Background technique [0002] Fibroblasts (fibroblast) are the main cell components of loose connective tissue, and the cells are spindle-shaped or flat star-shaped, with protrusions. According to different functional activity states, cells are divided into fibroblasts and fibroblasts: fibroblasts are cells with vigorous functional activities, with large cells and nuclei, clear outlines, large and prominent nucleoli, and weakly basophilic cytoplasm Sex, with obvious protein synthesis and secretion activities; fibrocytes (fibrocyte) functional activities are not active, the cell outline is not obvious, the nucleus is small and darkly stained, the nucleolus is not obvious, and the cytoplasm is less. These two types of cells can transform into each other. [0003] Different types of connective tissue contain different numbers of fibroblasts. Usually, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 陈海佳王一飞葛啸虎吴子杰王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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