A circulating type single-cell capturing chip

A single-cell, circulating technology, applied in the field of microfluidics, can solve the problems of high demand for cell input and difficulty in application, and achieve the effect of ensuring flushing

Active Publication Date: 2016-03-30
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve a certain number of captured single cells, it is necessary to input a large number of cells. In this literature, the number

Method used

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  • A circulating type single-cell capturing chip
  • A circulating type single-cell capturing chip
  • A circulating type single-cell capturing chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Figure 1~4 Shown is a schematic structural diagram of a circulating single-cell capture chip according to Example 1 of the present invention, and the chip is composed of three structural layers stacked one above the other. The bottom layer is the fluid flow layer 110, the upper layer is the pneumatic control layer 120, and there is an elastic membrane 130 between the two layers. The liquid flow layer 110 has a circulating liquid flow pipeline 1101, a liquid flow input pipeline 1102, and a liquid flow output pipeline 1103, each of which is 100 microns wide and 20 microns high. Both the liquid flow input pipeline 1102 and the liquid flow output pipeline 1103 have interfaces with The off-chip is connected, and there are multiple sets of protruding micropillars 1301 at the bottom of the liquid flow channel 1101. Each micropillar has a diameter of 10 microns, a height of 15 microns, and a gap of 4 microns between the micropillars to block single cells. The pneumatic contro...

Embodiment 2

[0049] Figure 5~7 It is a schematic diagram of the structure of the circulating single cell capture chip in Example 2 of the present invention, and the chip is composed of three structural layers stacked one above the other. The bottom layer is the fluid flow layer 110, the upper layer is the pneumatic control layer 120, and there is an elastic membrane 130 between the two layers. The liquid flow layer 110 has a circulating liquid flow pipeline 1101, a liquid flow input pipeline 1102, and a liquid flow output pipeline 1103, each of which is 100 microns wide and 20 microns high. Both the liquid flow input pipeline 1102 and the liquid flow output pipeline 1103 have interfaces with On the side of the elastic membrane 130 facing the liquid flow channel 1101, there are multiple groups of protruding micropillars 1301, each micropillar is 10 microns in diameter and 15 microns in height to block single cells. There are four pneumatic control pipes 1201, 1202, 1203, 1204 in the pneum...

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Abstract

A circulating type single-cell capturing chip is disclosed. The chip comprises a liquid layer, microsieves and a micropump, wherein the liquid layer comprises a circular liquid path, the liquid path is communicated with outside of the chip through a liquid feeding pipe and a liquid discharging pipe, the liquid feeding pipe and the liquid discharging pipe are respectively provided with a microvalve for isolating the liquid path from the outside, the microsieves are at least used for intercepting single cells in liquid flow flowing through the liquid path, and the micropump is at least used for driving the liquid flow to flow in the liquid path in a one-way and circulating manner. By utilization of the chip, single cells can be effectively captured from the liquid flow containing a small number of cells, the ratio of the number of the microsieves capturing the single cells reaches 90%, and the capturing efficiency is far higher than that in the prior art.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to a circulating single cell capture chip. Background technique [0002] With the continuous development of biotechnology research methods, the research level of biology is deepening and expanding from cell population to single cell level. Each cell is unique in time and space. Although they may come from the same ancestor, different time and space environments determine their specific genetic expression, resulting in different biological traits, which have great impact on evolution, drug resistance, and gene expression. Such research is of great value. However, analysis methods based on cell populations often conceal the differences between different cells within the population, ignoring these small but important information. Therefore, there is an urgent need to develop single-cell-based culture and analysis methods to study the differences between individual cells and und...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 甘明哲陈立桅
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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