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Nucleic acid amplification device, nucleic acid amplification device, and nucleic acid amplification method

A nucleic acid amplification device and nucleic acid amplification reaction technology, applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of large-scale systems and high costs

Inactive Publication Date: 2020-09-04
PANASONIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of using an external pump, manual work is required to connect the pump to the flow path, resulting in problems such as system size increase or cost increase.

Method used

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  • Nucleic acid amplification device, nucleic acid amplification device, and nucleic acid amplification method
  • Nucleic acid amplification device, nucleic acid amplification device, and nucleic acid amplification method
  • Nucleic acid amplification device, nucleic acid amplification device, and nucleic acid amplification method

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Effect test

Embodiment approach 1

[0096] first use Figure 1 to Figure 4 The configuration of the nucleic acid amplification device 1 according to Embodiment 1 of the present invention will be described. figure 1 It is a perspective view of the nucleic acid amplification device according to Embodiment 1 of the present invention, figure 2 is an exploded oblique view of the nucleic acid amplification device, image 3 is the plan view of the nucleic acid amplification device, Figure 4 is a cross-sectional view of the nucleic acid amplification device.

[0097] like Figure 1 to Figure 4 As shown, the nucleic acid amplification device 1 according to this embodiment is a device (device chip) for amplifying a target nucleic acid as a sample, and includes an introduction part (inlet) 120 for feeding a reaction solution containing at least a target nucleic acid Introduction; the nucleic acid amplification reaction part 110 is used to amplify the target nucleic acid contained in the reaction solution introduced i...

Embodiment approach 2

[0246] Next, use Figure 18A as well as Figure 18B The configuration of the nucleic acid amplification device 2 according to Embodiment 2 of the present invention will be described. Figure 18A is a plan view of the nucleic acid amplification device according to Embodiment 2 of the present invention, Figure 18B is an enlarged plan view of a mixing portion in the nucleic acid amplification device.

[0247] like Figure 18A as well as Figure 18B As shown, the nucleic acid amplification device 2 in the present embodiment is configured such that a plurality of introduction parts 120 of the nucleic acid amplification device 1 in the first embodiment are provided.

[0248] Specifically, the nucleic acid amplification device 2 has a first introduction part 120a and a second introduction part 120b, and a mixing part 150 is also provided between the first introduction part 120a, the second introduction part 120b and the nucleic acid amplification reaction part 110. .

[0249] ...

Embodiment approach 3

[0278] Next, use Figure 24A , Figure 24B as well as Figure 25 , the configuration of the nucleic acid amplification device 3 according to Embodiment 3 of the present invention will be described. Figure 24A It is a perspective view of the nucleic acid amplification device according to Embodiment 3 of the present invention, Figure 24B A schematic configuration of the nucleic acid amplification device is shown. Figure 25 is an exploded oblique view of the nucleic acid amplification device.

[0279] like Figure 24A to Figure 25 As shown, the nucleic acid amplification device 3 in the present embodiment is configured to further include the transport and retention unit 160 in addition to the nucleic acid amplification device 1 in the first embodiment.

[0280] The conveyance retention unit 160 is a conveyance retention region for stagnating the reaction solution. In other words, the transport retention unit 160 is not a transport advancing unit that keeps the head porti...

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Abstract

The nucleic acid amplification device (1) is provided with: an introduction part (120), into which a reaction solution containing target nucleic acid is introduced (120); a nucleic acid amplification reaction part (110), at least two or more temperatures with different temperatures region, and amplify the target nucleic acid contained in the reaction solution introduced into the introduction part (120); The flow path (100) has a capillary force transport mechanism for transporting the reaction solution using capillary force.

Description

technical field [0001] The present invention relates to a nucleic acid amplification device, a nucleic acid amplification device and a nucleic acid amplification method using the nucleic acid amplification device. Background technique [0002] A nucleic acid amplification device is a device for amplifying a target nucleic acid as a sample, for example, by repeatedly performing a desired temperature change on a reaction solution (reaction fluid) containing the target nucleic acid to amplify the target nucleic acid. [0003] Also, the use of microfluidic devices is well known as a method of speeding up the temperature change with respect to the reaction solution. A microfluidic device is a device capable of reacting a reaction solution containing a very small amount of sample or reagent, such as a micro reaction device (microreactor) or an integrated DNA device, a micro electrophoresis device, and the like. [0004] For example, Patent Document 1 and Non-Patent Document 1 dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/686
CPCB01L3/502715B01L7/525B01L2200/10B01L2300/0816B01L2300/0883B01L2300/1805B01L2300/1827B01L2300/0864B01L2300/0867B01L2400/0406B01L2400/086B01F25/4314B01F25/431971B01F25/4331B01F33/30C12Q1/686C12Q1/6806B01L2300/161B01L2300/165B01L2300/0654B01L2300/18B01L2200/06
Inventor 橘宏明涩谷章吾岩本成正民谷荣一斋藤真人辻幸司
Owner PANASONIC CORP