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A method for inducing somatic embryos from the embryo

A technology of somatic embryos and eel algae, which is applied in the field of plant tissue culture research, can solve problems that have not been reported, and achieve the effects of long acquisition time, increased ratio, and improved surface disinfection effect

Inactive Publication Date: 2017-10-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In the present invention, the embryos of Z. chinensis are used as explants, and the somatic embryos are induced by tissue culture technology. At present, no relevant patents and documents using this technical solution have been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Embodiment: a kind of method for inducing the somatic embryo of Zinaria chinensis, its implementation step comprises:

[0017] 1) Selection of explants: collect the healthy spathe inflorescences when the fruit of the eel is almost mature, peel off the bract leaves outside the spathe inflorescences, leave only the inflorescences inside and the fruit on them, and place them in the Soak and sterilize with 15% hydrogen peroxide in the ultra-clean workbench for 20 minutes, then rinse with sterilized sea water 3-5 times to obtain sterile inflorescences, remove the fruits on the inflorescences one by one, cut open the peel, and take out the sterile embryos;

[0018] 2) Induction culture of somatic embryos: Inoculate the sterile embryos in the induction medium under sterile conditions, and the entire culture process is carried out at 18-20°C and in the dark. Transfer once every half month. The induction medium was based on MS medium, supplemented with hydrolyzed casein 500 mg / ...

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PUM

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Abstract

The present invention relates to a method for inducing somatic embryos of Z. chinensis. The embryos of Z. chinensis are used as explants, and the sterile embryos are peeled off from fruits or seeds, and inoculated on a somatic embryo induction medium. 15-25°C, dark or weak light conditions for induction culture, transfer once every half month, after 60 days, small protrusions will appear on the embryos, forming somatic embryos, during which they will not go through the callus stage. The somatic embryo induction medium is a solid medium with MS medium as the base medium and supplemented with organic additives, carbon source, auxin and sea salt. The method of the present invention can directly induce the somatic embryos of Zinaria spp. Establishment provides another possible route and also lays the foundation for the success of seagrass tissue culture.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for inducing cell embryos with the somatic embryos of Z. eosinophila as explants. Background technique [0002] Eelweed ( Zostera marina L.) is the dominant species of seagrass fields along the northern coast of my country (Shandong, Hebei and Liaoning), and has extremely important ecological service functions and economic value: it is not only a good place for many economic fish and other marine economic organisms to inhabit, reproduce and hide , to protect the larvae and eggs of these marine organisms, and it can also form a large number of humus, providing excellent natural bait for plankton, young fish and shrimp and other marine animals, such as in Jiaodong Peninsula and Liaodong Peninsula, eelgrass seagrass field It is the best natural habitat for sea cucumber, a famous seafood. [0003] In the past two decades, the ecosystem of eelgrass se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑凤英李乐乐刘雪芹金艳梅张伟陈玉
Owner SHANDONG UNIV
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