Microfluidic separation device and method

Abstract

The invention discloses a microfluidic separation device and method, and belongs to the field of microfluidic. The device and method are used for separating mixed neutral particles in an electrolyte solution. The device comprises a first fluid driving micropump, a separation unit and a second fluid driving micropump. The first fluid driving micropump comprises first fluid driving microelectrodes used for driving an electrolyte solution inside the first fluid driving micropump to transversely flow at first preset flow speed, the separation unit comprises sample separation microelectrodes used for generating an uneven electric field, the second fluid driving micropump comprises second fluid driving microelectrodes used for driving an electrolyte solution inside the second fluid driving micropump to flow transversely at second preset flow speed, and the second preset flow speed is distributed in the longitudinal direction in a gradient mode. The microfluidic separation device and method achieve transportation and separation of the mixed neutral particles, no valves and no mechanical components are used in the transportation and separation process, the cost is low, and miniaturization of the microfluidic separation device is facilitated.

Description

technical field [0001] The invention relates to the field of microfluidics, in particular to a microfluidic separation device and method. Background technique [0002] Miniaturized Total Analysis System (μ-TAS), also known as "Lab-on-a-chip (Lab-on-a-chip)", is the pretreatment of samples, especially neutral particles, such as cells, colloid particles, etc. Routine laboratory operations such as transportation, separation, and detection are integrated on a chip with a size of square centimeters, which has significant advantages such as high analysis efficiency, reduced sample consumption, small volume, and easy integration. Microfluidic chip is a hot field in the development of "lab-on-a-chip" and has broad application prospects. field. Among them, the transportation and separation of samples are the key links of analysis and detection, and their integration in microfluidic chips is of great significance for realizing the miniaturization, automation and portability of biolo...

AI Technical Summary

Problems solved by technology

In the existing microfluidic chips, the injection and transportation of mixed neutral particle samples are realized by micro-syringe pumps, which is costly and not conducive to the integration with separation units to realize microfluidic separation miniaturization

Images