Traditional Chinese medicine composition for treating cancer and cancer pain and fevers
A cancer pain and composition technology, applied in the field of medicine, can solve the problems of affecting the quality of life of patients, high toxic and side effects, strong addiction, etc., and achieve the effect of low cost, small toxic and side effects, and quick results
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[0012] Weigh 50 grams of Ganoderma lucidum, 50 grams of ginseng, 100 grams of astragalus, 30 grams of Atractylodes macrocephala, 20 grams of atractylodes rhizome, 30 grams of poria cocos, 30 grams of coix seed, 20 grams of red bean, 30 grams of Chinese yam, 20 grams of Hangshao, 15 grams of cassia twig, salvia miltiorrhiza 30g, Danpi 20g, Shanzhi 20g, Angelica 20g, Chuanxiong 20g, Scutellaria baicalensis 20g, Corydalis 20g, Polygala 20g, Phellodendron 20g, Porcupine thorn 20g, Digupi 20g, Albizia bark 20 grams, bellflower 10 grams, Aescin chinensis 20 grams, Nansha ginseng 20 grams, agrimony 20 grams, Bupleurum 30 grams, frankincense 20 grams, myrrh 20 grams, Jianzhongxiao 20 grams, small copper hammer 20 grams, 20 grams of saponins, 20 grams of kudiding, 50 grams of baiyaozi and 50 grams of xanthazolium, pulverized, passed through a 100-mesh sieve, and mixed uniformly to obtain a powder.
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[0014] MTT (purchased from Sigma Company) was dissolved in normal saline, prepared into a working solution of 5 mg / mL, and stored at -20°C; dimethylsulfoxide (DMSO) was produced by Xi'an Chemical Reagent Factory and was analytically pure. Human breast cancer T47D cells were purchased from Shanghai Fengshou Biotechnology Co., Ltd.
[0015] Principle of MTT method
[0016] MTT is an acceptable H + yellow dye. In the mitochondrial respiratory chain of living cells, succinate dehydrogenase and cytochrome C can cleave the tetrazolium ring of MTT to produce blue-purple formazan crystals. Dead cells do not contain this dehydrogenase. The amount of Formazan crystals generated is directly proportional to the number of viable cells, and the DMSO solution of the crystals has a maximum absorption peak at 570nm. Therefore, the effect of the drug on cell proliferation can be evaluated by detecting the amount of formazan crystals.
[0017] Human breast cancer T47D cells were selected as ...
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