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A kind of technique of producing dark tea by inoculation method

A technology, dark tea technology, applied in the field of microorganisms and food biology, can solve the problems of uncontrollable golden flower quantity and unstable quality of dark tea products, and achieve simple and feasible inoculation process, significant and stable flower quantity Effect

Active Publication Date: 2019-05-31
中国土产畜产进出口有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention lies in the problems in the prior art that the quality of dark tea products is unstable and the number of golden flowers is uncontrollable, and then a kind of inoculation method with stable quality of dark tea products and controllable number of golden flowers is provided. The process of producing dark tea is to realize the selection and breeding of good strains, the preparation of good seeds, the selection of good links, and the production of good black tea

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  • A kind of technique of producing dark tea by inoculation method
  • A kind of technique of producing dark tea by inoculation method
  • A kind of technique of producing dark tea by inoculation method

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Experimental program
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Effect test

Embodiment 1

[0058] The separation and purification of embodiment 1 bacterial strain

[0059] The separation and purification steps of the dominant "Golden Flower" bacteria of the dark tea are as follows:

[0060] (1) Select tea sample

[0061] Select Hunan Anhua Tea Factory The Fuzhuan brick tea products of different years in the tea leaves in the wooden warehouse and the mainstream Fuzhuan brick tea products produced and sold in Yiyang City, Hunan Province were used as materials for the isolation of the original strains. The tea samples of the above selected products meet the requirements of GH / T 1070 on tea packaging, meet the requirements of GH / T1071 on tea storage, meet the limits of pollutants in food in GB 2762-2012, and meet the maximum residues of pesticides in GB2763 / GB26130 The limited amount meets the relevant standards of GB / T9833.2-2013 on "Compressed Tea"; at the same time, the selected tea samples meet: no damage to the packaging, no stains; The same batch of products, ...

Embodiment 2

[0077] The breeding of embodiment 2 bacterial strains

[0078] Breeding step a. Breeding strains according to the growth degree of the "golden flower" in the tea according to the characteristics of bacteria in the tea leaves during fermentation and the growth degree of golden flowers

[0079] Breeding ideas: Under the same culture conditions, the same batch of black hair tea raw materials from the same production area were selected for the fermentation test of strain inoculation. If the strain can meet the requirements of tea quality for the strain characteristics of fermenting bacteria and the growth degree of "Golden Flower" for the strain, then after the inoculation of the strain, the strain can grow rapidly in tea products and within a certain period of time The fermented tea leaves can reach a certain number of bacteria, so as to grow into dominant bacteria and inhibit the growth of miscellaneous bacteria; and its good characteristics are reflected in the appearance and q...

Embodiment 3

[0098] Embodiment 3 bacterial classification property identification

[0099] Morphological, physiological and biochemical characteristics, and molecular biological characteristics of Eurotium cristatum NHRI-BC-1.5.1 obtained in the above-mentioned embodiment 2 are identified, specifically as follows:

[0100] A. Morphological characteristics of bacteria

[0101] Take the strain numbered NHRI-BC-1.5.1, inoculate the strain on the PDA solid plate medium by the streak culture method, and cultivate it in a constant temperature incubator at 28°C for 15 days, and pick the medium when observing the morphology of the bacteria The mycelium on the colony was observed under an optical microscope with a magnification of 10 times the objective lens and 16 times the eyepiece.

[0102] Observation results showed that the bacteria were filamentous, septated and light yellow in color.

[0103] B. Colony Morphological Characteristics

[0104] Take the strain numbered NHRI-BC-1.5.1, inoculat...

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Abstract

The present invention belongs to the technical field of microorganisms and food biology, and particularly relates to a process for producing a dark tea by inoculating bred Eurotium cristatum, wherein the process comprises that Eurotium cristatum with a classification name of Eurotium cristatum NHRI-BC-1.5.1, preserved in the China General Microbiological Culture Collection Center, and with a preservation number of CGMCC No.8730 is adopted as a fermentation strain, and inoculation is performed after stacking or before vapor steaming or after metering step and before secondary vapor steaming. According to the present invention, the prepared dark tea product has characteristics of high quality and good stability, and the controllability of the Eurotium cristatum fermentation is basically achieved so as to further achieve the standardized control of the dark tea product.

Description

technical field [0001] The invention belongs to the field of microorganism and food biotechnology, and in particular relates to a process for producing dark tea by inoculation. Background technique [0002] Black tea is one of the six traditional teas (green tea, black tea, white tea, green tea, dark tea and yellow tea), and it is also a unique tea in my country. Common dark teas include Anhua dark tea, Yunnan Pu'er tea, Guangxi Liubao tea, Hubei old green tea, etc. Among them, the traditional Anhua dark tea mainly uses the large-leaf species in the tea area of ​​Xuefeng Mountains in Hunan as raw materials. Alcoholic or slightly astringent, orange-yellow soup, slightly unique quality characteristics of rosin black hair tea and the general term for products refined and processed from it. It is named after it is produced in Anhua County, Hunan, China. Anhua dark tea contains bioactive ingredients such as tea polyphenols and their metabolites, polysaccharides, various amino ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23F3/10A23F3/06
Inventor 王春玲李颂刘志伟卢建明许衡陈晓阳姚呈祥王大量王伟张明明林可黄红芳
Owner 中国土产畜产进出口有限责任公司
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