Orlistat rapid detection method

A detection method and orlistat technology, applied in the field of analytical chemistry detection, can solve the problems of expensive equipment, high operating costs, and high operator requirements, and achieve the effects of simple operation, improved sensitivity, and obvious phenomena

Active Publication Date: 2016-04-20
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although the above method can achieve the purpose of detection, the equipment is expensive, the operating cost is high, and the requirements for operators are also high, which is not conducive to widespr...

Method used

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: the detection of tea preparation

[0032] Reagent preparation: the extract is petroleum ether (60-90°C); the washing solution is 70% (v / v) ethanol aqueous solution; the hydroxylamine hydrochloride solution is 3.5wt% hydroxylamine hydrochloride-70% (v / v) ethanol-water solution; The alkali solution is 1mol / L sodium hydroxide-70% (v / v) ethanol-water solution; the acidification solution is 1mol / L hydrochloric acid aqueous solution; the color developing solution is 9wt% ferric chloride aqueous solution.

[0033] Sample: Banshengyuan Tianxin Slimming Tea (tea preparation, the labeled dosage is 1 bag, and this product does not contain orlistat as determined by LC-MS / MS method.)

[0034] The detection method, the steps are:

[0035]Take 1 bag of the above-mentioned sample to be tested, grind the contents finely, take 1g of powder, add 2mL of petroleum ether (60-90°C), shake fully for 3min, let the layers stand for 4min, take 2mL of the supernatant, and then add 2...

Embodiment 2

[0037] Embodiment 2: the detection of capsule

[0038] Reagent preparation: extracting solution is n-hexane; washing solution is 80% (v / v) ethanol solution; hydroxylamine hydrochloride solution is 4.0wt% hydroxylamine hydrochloride-80% (v / v) ethanol-water solution; strong base solution is 1.5mol / L sodium hydroxide-80% (v / v) ethanol-water solution; acidifying solution is 1.5mol / L nitric acid aqueous solution; color developing solution is 12wt% ferric chloride aqueous solution.

[0039] Sample to be tested: Shuerjia Orlistat Capsules (capsules, the labeled dose is 1 capsule, and the LC-MS / MS method determines that this product contains orlistat.)

[0040] The detection method, the steps are:

[0041] Take 0.5g of the above-mentioned sample powder to be tested, add 1mL of n-hexane, shake fully for 4min, let the layers stand for 5min, take the supernatant, then add 1mL of 80% (v / v) ethanol solution, shake gently for 2min, Set the layers for 5 minutes, take the supernatant, add ...

Embodiment 3

[0042] Result analysis: the color of the sample solution to be tested is wine red, indicating that the sample contains orlistat, and the result is consistent with the actual situation. Embodiment 3: the detection of tablet

[0043] Reagent preparation: extracting solution is cyclohexane; washing solution is 60% (v / v) methanol aqueous solution; hydroxylamine hydrochloride solution is 3.0wt% hydroxylamine hydrochloride-60% (v / v) ethanol-water solution; strong alkali solution is 0.5mol / L sodium hydroxide-70% (v / v) ethanol-water solution; the acidifying solution is 0.5mol / L sulfuric acid aqueous solution; the color developing solution is 6wt% ferric chloride aqueous solution.

[0044] Sample to be tested: Xiumeier Slimming Effervescent Tablets (tablets, the indicated dosage is 1 tablet, and this product does not contain orlistat as determined by LC-MS / MS method.)

[0045] The detection method, the steps are:

[0046] Take 0.5g of the above-mentioned sample powder to be tested, ...

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Abstract

The present invention discloses an orlistat rapid detection method, a to-be-tested sample is extracted, treated with a washing solution, reacted with a hydroxylamine hydrochloride solution and a strong alkali solution for extraction, and then acidified for coloration, and whether orlistat is contained can be judged by color. The detection method is simple and low in cost for analysis, the to-be-tested sample is pretreated, and then reacted with a detection agent, so that the interference of relevant factors can be eliminated, observation of the color of the solution is facilitated, and whether the orlistat is contained in the sample can be judged more accurately. The detection method is strong in specificity, interfering substances can be eliminated by extraction, washing, extraction and separation, the sensitivity of the coloration reaction is increased, test result correct rate is high, the coloration reaction is rapid, the phenomenon is obviously, and the method is suitable for on-site rapid detection.

Description

technical field [0001] The invention belongs to the field of analytical chemistry detection and relates to a rapid detection method of orlistat. Background technique [0002] In recent years, with the improvement of people's living standards, the obesity problem caused by factors such as unreasonable diet structure and lack of exercise has become increasingly serious. According to the World Health Organization, 39% of adults aged 18 and over were overweight and 13% were obese in 2014. Obesity can induce or aggravate diabetes, fatty liver, cardiovascular and cerebrovascular diseases, sleep apnea syndrome, musculoskeletal disorders, and certain cancers, and has become one of the ten major threats to human health. [0003] Along with people's urgent need for weight loss, various weight loss products are also increasing day by day, and good and bad people are mixed. Rapid weight loss with chemical drugs usually brings obvious adverse reactions, while health care products, food...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 咸瑞卿石峰巩丽萍梁翠荣王蕾蕾郭常川邢晟姜树银由鹏飞
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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