Universal PCR detection primers and detection method for vertebrate-derived ingredients

Abstract

The invention discloses universal PCR detection primers and a detection method for vertebrate-derived ingredients. The sequences of the primer pair are as shown in F-primer: 5'-TGTTTACCAAAAACATCACCTCCA-3', and R-primer: 5'-AGTTAAAGCTCCATAGGGTCT-3'. According to the invention, the pair of universal primers is designed in a highly conserved domain in accordance with various vertebrate mitochondrion DNA (mtDNA) sequence revealed in GenBank, and the universal PCR detection method is established; experimental results show that the primers are relatively strong in university, and effective amplification on all collected samples is achieved; and in accordance with sequencing results, the effective identification of species is achieved.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a universal PCR detection primer and a detection method for components derived from vertebrates. Background technique [0002] In the halo of modern civilized society, human beings are driven by interests to illegally hunt and kill all kinds of animals repeatedly, which seriously damages the ecological balance. The phenomenon of counterfeiting traditional Chinese medicinal materials and skins of rare animal products in commodity transactions has also continued unabated, seriously damaging the rights and interests of consumers. From the perspective of animal disease prevention and control, my country has also issued a number of relevant laws and regulations on the breeding, production and processing of animal agricultural and sideline products. The prevention and control of scrapie and sheep stipulates that the addition and use of animal feed in ruminant fe...

AI Technical Summary

Problems solved by technology

Due to the complex biological components of vertebrates and the various chemical components of related animal products, and containing a large amount of similar components such as protein and fat, the results of using general sensory and physical and chemical identification methods are not satisfactory. Due to their own inadequacies, it is impossible to accurately or conveniently identify

PCR technology is widely used at home and abroad for its high specificity and sensitivity, but most of the existing detection methods are limited to one or several methods of animal-derived components, and the research on general detection and identification technologies for vertebrates is still relatively limited. rare

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