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Application of exosome to preparing of acute-hepatic-failure treating medicine and medicine composition

An acute liver failure, exosome technology, applied in the field of biomedicine, can solve problems such as poor prognosis

Inactive Publication Date: 2016-05-04
ZHEJIANG SHENGCHUANG PRECISION MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is often induced by hepatitis virus infection, drugs and poisons, etc. The prognosis is extremely poor, and there is currently no effective treatment except liver transplantation

Method used

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  • Application of exosome to preparing of acute-hepatic-failure treating medicine and medicine composition
  • Application of exosome to preparing of acute-hepatic-failure treating medicine and medicine composition
  • Application of exosome to preparing of acute-hepatic-failure treating medicine and medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] refer to figure 1 As shown in the flowchart, the method of patent document CN201510112156.3 is used to isolate, cultivate and identify menstrual blood mesenchymal stem cells, and the isolated menstrual blood mesenchymal stem cells 2 and cultured in a saturated humidity incubator. Select passage 3-6 menstrual blood mesenchymal stem cells in a good growth state and culture them in αMEM medium containing 15% by volume of fetal bovine serum. When the cells are fused to 70-80%, change to serum-free αMEM medium for culture. Collect and culture after 24 hours supernatant. The collected menstrual blood mesenchymal stem cell supernatant was centrifuged at 4°C, 2000×g for 10 min to remove cell debris, and the supernatant from which cell debris was removed was filtered through a 0.22 μm filter to remove cysts with larger diameters. Then transfer the filtered supernatant to a 30kDa MWCO ultrafiltration centrifuge tube, centrifuge at 4000×g for 1h at 4°C to concentrate, and slowly...

Embodiment 2

[0043] This example demonstrates the therapeutic effect of exosomes secreted by blood mesenchymal stem cells on acute liver failure.

[0044] Mice with a body weight of 20 g were injected intraperitoneally with D-galactosamine (D-GalN) and lipopolysaccharide (LPS) to establish an animal model. The survival mental state of mice, and successfully established a mouse model of acute liver failure.

[0045] In the tail vein of each mouse model of acute liver failure, a dose of 1 μg / μL PBS dispersed exosomes secreted by blood mesenchymal stem cells was injected into the tail vein for treatment. The acute liver failure model injected into mice was the positive drug group.

[0046] Detect aspartate aminotransferase (AST) and alanine aminotransferase (ALT) indexes in serum of mice with acute liver failure, the results are as follows: Figure 4 As shown, it can be seen that compared with the D-GalN / LPS model control group, exosomes derived from menstrual blood-derived mesenchymal stem...

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Abstract

The invention provides an application of exosome to preparing of acute-hepatic-failure treating medicine. The exosome is an exosome secreted through personal-menstrual-blood mesenchymal stem cells. The invention further provides a medicine composition, wherein the medicine composition comprises the exosome secreted through the personal-menstrual-blood mesenchymal stem cells and carriers acceptable in pharmacy. According to the medicine composition in the technical scheme, the survival time of an acute-hepatic-failure animal model can be prolonged, the bleeding rate and the necrotic rate of the livers can be decreased, and secreting of inflammatory factors through liver cells can be promoted, so that acute hepatic failure is effectively treated, and prognosis of an acute-hepatic-failure patient is improved accordingly.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the use and pharmaceutical composition of exosomes in the preparation of medicines for treating acute liver failure. Background technique [0002] Vesicles expelled by cells include microvesicles, exosomes, ectosomes, apoptotic vesicles, etc. Wherein, the diameter of microvesicles is 100-1000 nm. The diameter of exosomes is 20-100nm, which is a uniform vesicle-like body that cells actively secrete to the outside of the cell, and has physiological functions such as anti-tumor immunity and promoting angiogenesis. Different types of cells can release different exosomes, and exosomes can also be isolated from blood, urine, breast milk and bronchial lavage fluid under normal physiological conditions. In the past few decades, a large number of studies have found that all human body fluids have exosomes, because they contain specific protein, nucleic acid and fat components, so they...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P1/16C12N5/0775
CPCA61K35/28C12N5/0665
Inventor 余艳春
Owner ZHEJIANG SHENGCHUANG PRECISION MEDICAL TECH CO LTD
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