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A kind of ammonia nitrogen degrading bacterial agent preservative and preservation method
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A technology for degrading bacteria and preservatives, which is applied in the field of preservatives for ammonia-nitrogen degrading bacterial agents in low-temperature storage. It can solve the problems of serious bacterial contamination, reduced metabolic activity, and low survival rate, and achieve stable metabolic activity, wide sources, and low prices. Effect
Active Publication Date: 2019-10-01
TIANJIN CARING TECH DEV
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[0005] The purpose of the present invention is to solve the problems of low survival rate, serious contamination of miscellaneous bacteria, and reduced metabolic activity in the preservation of live ammonia nitrogen degrading bacteria preparations, and provide a low-temperature preservation method with high storage efficiency, good adaptability and low cost for ammonia nitrogen degrading bacteria preparations. Preservatives and preservation methods to ensure that they have a high number of viable bacteria and strong metabolic activity during application
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Embodiment 1
[0025] In this embodiment, the composition of the ammonia nitrogen degrading bacterial agent preservative is: 0.08g of nitrite, 0.15g of D-isoascorbic acid, 4mL of glycerin, 0.38g of dihydrogen phosphate, 2g of dihydrogen phosphate and appropriate amount of water.
[0026] Dissolve the above-mentioned substances except glycerol in water to make aqueous solutions, mix the aqueous solutions, then add glycerin, and then supplement water to 1L, and then the ammonia nitrogen degrading bacterial agent preservative is prepared.
Embodiment 2
[0028] In this embodiment, the composition of the ammonia nitrogen degrading bacterial agent preservative is: 0.15 g of sodium nitrite, 0.1 g of D-isoascorbic acid, 8 mL of glycerin, 0.25 g of sodium dihydrogen phosphate, 4.1 g of dipotassium hydrogen phosphate and an appropriate amount of water.
[0029] Dissolve the above-mentioned substances except glycerol in water to make aqueous solutions, mix the aqueous solutions, then add glycerin, and replenish water to 1L to prepare the ammonia nitrogen degrading bacterial agent preservative.
Embodiment 3
[0031] In this embodiment, the composition of the ammonia nitrogen degrading bacterial agent preservative is: 0.23g of nitrite, 0.05g of D-isoascorbic acid, 12mL of glycerin, 0.13g of dihydrogen phosphate, 6g of dihydrogen phosphate and appropriate amount of water.
[0032] Dissolve the above-mentioned substances except glycerol in water to make aqueous solutions, mix the aqueous solutions, then add glycerin, and replenish water to 1L to prepare the ammonia nitrogen degrading bacterial agent preservative.
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Abstract
The invention discloses a preservative for an ammonia nitrogen degrading microbial inoculum. The preservative comprises water, an inhibitor, an antioxidant, a cell protective agent and inorganic salt modifiers, wherein the inhibitor is nitrite, the cell protective agent is glycerin and the inorganic salt modifiers are dihydric phosphate and hydrophosphate. The invention also discloses a preserving method of the ammonia nitrogen degrading microbial inoculum, wherein the method adopts the preservative to preserve the microbial inoculum. Compared with other preserving methods in the prior art, the preservative and the preserving method are advantageous in that 1) the preservative is advantaged by wide sources of components, low prices and relatively simpler and more convenient preservation, easy operation, no secondary pollution, and the like, and 2) during preservation of the microbial inoculum, contamination is effectively prevented and viable bacterium cells of a nitrococcus microbial inoculum are free of harm, metabolic activity is stable after preservation, the need of applying the microbial inoculum for reinforcing nitration functions in sewage treatment systems can be met, and the preservative and the preserving method are high in application value.
Description
technical field [0001] The invention relates to the technical field of environmental protection, in particular to a preservative and a preservation method for low-temperature preservation of ammonia nitrogen degrading bacteria agent, through the protective effect of the preservative, to prevent the ammonia nitrogen degrading bacteria agent from polluting miscellaneous bacteria for a long time and at low cost, The purpose of maintaining nitrification activity. Background technique [0002] Water resources are an important part of the human environment, a medium for energy flow and material circulation in the ecosystem, a precious resource on the earth, and one of the necessary elements for the growth and survival of organisms. [0003] At present, the problem of water environment pollution in my country is still very serious, among which the problem of ammonia nitrogen is particularly prominent. Ammonia nitrogen is one of the important indicators for controlling harmful subst...
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