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A medium for maintaining the growth of wild black powder fungus mycelium in vitro

A technology of mycelium and culture medium, which is applied in the field of culture medium for maintaining the growth of wild black powder mycelium in vitro

Active Publication Date: 2019-03-12
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that Ustilago sativa can be cultured in vitro and produce hyphae, the mycelia of Ustilago sativa cultured on YEPS medium (2% peptone, 2% sucrose, 1% yeast powder) are different from the filamentous Fungal hyphae are morphologically variable and incapable of long-term mycelial growth

Method used

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  • A medium for maintaining the growth of wild black powder fungus mycelium in vitro
  • A medium for maintaining the growth of wild black powder fungus mycelium in vitro
  • A medium for maintaining the growth of wild black powder fungus mycelium in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the medium and YEPS medium that the medium and YEPS medium carry out the comparative test of the cultivation of the mycelia of the mushroom Ustilago mycelium

[0015] Mycelia Growth Medium (GM) Formula for Ustilago smut (1 L): K 2 HPO 4 1 g, MgSO 4 ·7H 2 O 0.5 g, FeSO 4 ·7H 2 O 0.01g, KCl 0.5g, yeast powder 2.5g, peptone 5g, sucrose 3.42g, histidine 5.2g, agar 10g;

[0016] YEPS medium formula (1 L): 20g peptone, 20g sucrose, 10g yeast powder.

[0017] 1) The smut fungus in Zizania zizania was isolated by grinding method, and the purely cultured colonies were obtained after culturing on PDA solid medium at 28°C for 6 days ( figure 1 A), the colony has irregular edges, but no obvious white hyphae. right figure 1 The colonies in A were microscopically observed, and the irregular edges were found to be hyphae ( figure 1 B for figure 1 Microscopic observation of the colony indicated by the arrow in A), but the growth of mycelia in vitro cannot be ma...

Embodiment 2

[0020] Example 2: Screening of the main components of the medium (screening for the best carbon and nitrogen sources): sucrose, histidine

[0021] In order to screen the best mycelia growth carbon and nitrogen source, we in the basal medium (K 2 HPO 4 1 g, MgSO 4 ·7H 2 O0.5 g, FeSO 4 ·7H 2 O 0.01 g, KCl 0.5 g, agar 10 g, 1 L) were added with different carbon and nitrogen sources to detect their effects on mycelial growth, and to screen the components that could promote mycelial growth. In the screening process of carbon and nitrogen sources, galactose, glucose, fructose, sorbitol, mannitol, sucrose, lactose, and maltose were selected as carbon sources; potassium nitrate, ammonium nitrate, ammonium sulfate, sodium glutamate, methionine , arginine, urea, and histidine were used as nitrogen sources; the carbon source was in units of C6 and the concentration was 20 mm / L, and the nitrogen source was in units of N1 and the concentration was 100 mm / L. Using potassium nitrate a...

Embodiment 3

[0028] Take K 2 HPO 4 0.85 g, MgSO 4 ·7H 2 O 0.45g, FeSO 4 ·7H 2 O 0.008g, KCl 0.45g, yeast powder 2g, peptone 4g, sucrose 3g, histidine 4.5g, agar 10g adds 1L water and prepares and obtains and maintains the substratum that Ustilago smut mycelia grows in vitro.

[0029]Cultivate Ustilago smut into the above-mentioned medium, and observe the growth of mycelia through an optical microscope during 2 days of cultivation. The growth and maintenance of the mycelia on the medium are good. Continue to cultivate and observe. After 3 days, it can be clearly seen that the mycelium grows well After continuing to cultivate for 10 days, the colonies were observed with the naked eye to be covered with white aerial hyphae.

[0030] It can be seen that the medium can maintain the growth of Ustilago smut mycelium in vitro.

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Abstract

The invention discloses a culture medium capable of maintaining in-vitro growth of hyphae of ustilago esculenta, and belongs to the technical field of culture media. Every liter of the culture medium comprises the following components: 0.85 to 1.15 grams of K2HPO4, 0.45 to 0.55 gram of MgSO4.7H2O, 0.008 to 0.012 gram of FeSO4.7H2O, 0.45 to 0.55 gram of KCl, 2 to 3 grams of yeast powder, 4 to 6 grams of peptones, 3 to 4 grams of sucrose, 4.5 to 5.9 grams of histidine and 10 to 15 grams of agar. The culture medium capable of maintaining maintaining in-vitro growth of the hyphae of the ustilago esculenta is reasonable in compatibility; optimal carbon and nitrogen sources are added into a basic culture medium, so that in-vitro growth of the hyphae of the ustilago esculenta can be maintained by the culture medium.

Description

technical field [0001] The invention belongs to the technical field of culture medium, and in particular relates to a culture medium for maintaining the mycelium growth of Ustilago smut in vitro. Background technique [0002] Ustilago rugosa belongs to the Basidiomycotina genus Ustilago, is a typical dimorphic fungus, and is closely related to Ustilago zea. So far, Zizania is the only known host, and Zizania fertiliser is the result of the interaction between Zizania plants and the smut fungus that specifically parasitizes in the body. In the field, Zizania is threatened by various diseases and insect pests. The main diseases are flax spot and rust. Nowadays, pesticide control is still the main prevention and control measure for field diseases and insect pests. However, in the field application, it is found that the use of fungicides has a significant impact on the yield of Zizania zizania during the process of disease control. It will lead to the inability of wild rice st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 叶子弘张雅芬崔海峰俞晓平
Owner CHINA JILIANG UNIV
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