A kind of purification preparation method of red yeast lovastatin
A technology of lovastatin and red yeast rice, applied in the field of purification and preparation of red yeast rice lovastatin, can solve the problems of high cost, low yield, complex process, etc., and achieve the effect of less impurities, high purity, and complex process
Active Publication Date: 2017-12-08
FUZHOU UNIV
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Problems solved by technology
By adopting silica gel column and Sephadex LH20 column chromatography separation, and selecting different polar organic solvents for gradient elution, a very good purification effect (purity can reach 95%) is achieved, which solves the problem of complex existing processes, low yield and high cost The problem
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Embodiment 1
[0025] Separation and purification method of lovastatin in Monascus fermented liquid:
[0026] (1) Centrifuge the Monascus purple fermented liquid to remove the bacteria, take the supernatant and divide it into 250 mL Erlenmeyer flasks with a liquid volume of 200 mL, adjust the pH to 11 with 6 mol / L sodium hydroxide, and set Shake at 180 r / min for 1 h, take it out and centrifuge at 4200 r / min for 5 min, and take the supernatant; after testing, the lovastatin content in the supernatant is 350-400 mg / L;
[0027] (2) Take 120-mesh silica gel soaked in ethyl acetate and then wet-pack the column. The diameter of the column is 9mm, and the height of the column is about 12-13cm to obtain a silica gel column; take 0.5mL of the supernatant in step (1) and load it. After loading the sample, first add 15 mL of ethyl acetate for elution, then add 20 mL of mixed solvent 1 (ethyl acetate:methanol=2:1, v / v) for elution, and finally add 8 mL of mixed solvent 2 (ethyl acetate :methanol=3:1, v...
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Abstract
The invention belongs to the field of extraction and purification of medicine, in particular relates to a purification and preparation method of monascus-lovastatin. The purification and preparation method comprises the following steps: firstly separating a supernatant solution of a liquid fermentation solution of monascus by using a silica gel column, and carrying out gradient elution by adopting ethyl acetate, a solvent prepared from the ethyl acetate and methanol according to the volume ratio of 2:1 and a solvent prepared from the ethyl acetate and the methanol according to the volume ratio of 3:1 in sequence, thus obtaining a dissolved solution; then separating the dissolved solution by adopting an LH20 column, and carrying out gradient elution by adopting ultrapure water, 20 percent of methanol solution and 50 percent of methanol solution in sequence, thus obtaining a purified lovastatin solution. According to the purification and preparation method of the monascus-lovastatin, disclosed by the invention, a solvent with larger toxicity is prevented from being used, the separation efficiency is obviously increased (the purity can reach 95 percent), the problems of complicated technology and high cost are solved, and the purification and preparation method is suitable for industrial production.
Description
technical field [0001] The invention belongs to the field of extraction and purification of medicines, in particular to a method for the purification and preparation of red yeast lovastatin. technical background [0002] Lovastatin is a metabolite of microorganisms. It has been proven to have a strong inhibitory effect on cholesterol synthesis. It belongs to statin drugs. Its high efficiency and low cost make it the first choice for clinical treatment of high cholesterol and hyperlipidemia. . Its chemical name is [1S-[1α(R*), 3α, 7β, 8β(2S*, 4S*), 8αβ]]-1,2,3,7,8,8α-hexahydro-3,7- Dimethyl-8-[2-(tetrahydro-4-hydroxy-6-oxo-dihydropyran-2)-ethyl]-1-naphthyl-2-methylbutyrate, with two structures The forms are acid type and lactone type, which almost exist as ring-opening acid type under strong alkali conditions, and both forms exist under acidic conditions, and they can be transformed into each other. The chemical structure formula is as follows: [0003] [0004] At pres...
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IPC IPC(8): C07D309/30
CPCC07D309/30
Inventor 林娟李亮许鑫琦叶秀云
Owner FUZHOU UNIV