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Tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, method for preparing same and application of tea seed flavonoid aglycone and tea sapogenin zinc coordination complex

A technology of flavonoid aglycone and aglycon zinc, which is applied in the field of medicine, can solve problems such as unreported research, and achieve the effects of simple process, improved central nervous system protection activity, and convenient industrial production.

Inactive Publication Date: 2016-05-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Tea seed flavonoids and saponins are directly extracted without separation to prepare flavonoid aglycon and tea saponin zinc complexes, which have not been reported as new anti-neurodegeneration drugs

Method used

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  • Tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, method for preparing same and application of tea seed flavonoid aglycone and tea sapogenin zinc coordination complex
  • Tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, method for preparing same and application of tea seed flavonoid aglycone and tea sapogenin zinc coordination complex
  • Tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, method for preparing same and application of tea seed flavonoid aglycone and tea sapogenin zinc coordination complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Add 1 kg of defatted tea seed meal to 10 L of ethanol aqueous solution with a volume fraction of 80%, reflux extraction at 70° C. for 3 hours, filter, add hydrochloric acid to the filtrate until the final concentration of HCl is 2 mol / L, and hydrolyze at 80° C. for 5 hours. Concentrate under reduced pressure at 0.1 atmospheric pressure to 1 / 3 of the volume of the filtrate, add 3 times the volume of the concentrated solution to precipitate, and redissolve the precipitate with anhydrous ethanol equal to its mass, add 20 g of anhydrous sodium carbonate, 10 g of zinc acetate, React at 70°C for 10 hours, adjust the pH to 8 with ammonia water, let the precipitate stand for 24 hours, collect the precipitate, wash it with ethanol, and dry it in vacuum at 50°C for 6 hours to obtain 48 g of flavonoid aglycone and theasapogenin zinc complex.

[0035] The product was made into a 0.2mg / mL sample solution with DMSO as a solvent. The ultraviolet scanning showed that the characteristic ...

Embodiment 2

[0037] Add 1kg of degreased camellia seed meal to 15L of 70% ethanol aqueous solution, reflux extraction at 60°C for 2 hours, filter, add hydrochloric acid to the filtrate until the final concentration of HCl is 5mol / L, and hydrolyze at 70°C for 8 hours, at 50°C and 0.01 Concentrate under reduced pressure to 1 / 3 of the volume of the filtrate under 1 atmospheric pressure, add 5 times the volume of the concentrated solution to precipitate water, after the precipitate is redissolved with dehydrated alcohol of its equal mass, add 10 g of anhydrous sodium carbonate, 5 g of zinc acetate, 80 React at ℃ for 5 hours, adjust the pH to 9 with ammonia water, let the precipitation stand for 8 hours, collect the precipitate, wash it with ethanol, and dry it in vacuum at 80℃ for 3 hours to obtain 36 g of flavone aglycone and theasapogenin zinc complex.

[0038] The product is detected in the same manner as in Example 1, and the result is consistent with Example 1.

Embodiment 3

[0040] Add 1kg of degreased camellia seed meal to 12L of ethanol aqueous solution with a volume fraction of 85%, reflux extraction at 65°C for 1 hour, filter, add hydrochloric acid to the filtrate until the final concentration of HCl is 3mol / L, and hydrolyze at 80°C for 5 hours, at 60°C and 0.05 Concentrate under reduced pressure to 1 / 3 of the volume of the filtrate under 1 atmospheric pressure, add 4 times the volume of the concentrated solution to precipitate water, after the precipitate is redissolved with dehydrated alcohol of its equal mass, add 20 g of anhydrous sodium carbonate, 15 g of zinc acetate, 70 React at ℃ for 10 hours; adjust the pH to 10 with ammonia water, let the precipitation stand for 10 hours, collect the precipitate, wash it with ethanol, and dry it in vacuum at 60℃ for 4 hours to obtain 52 g of flavone aglycone and theasapogenin zinc complex.

[0041] The product is detected in the same manner as in Example 1, and the result is consistent with Example 1....

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Abstract

The invention belongs to the field of medicines, and discloses a tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, a method for preparing the same and application of the tea seed flavonoid aglycone and tea sapogenin zinc coordination complex. The method particularly includes steps of extracting degreased tea seed meal by the aid of ethyl alcohol aqueous solution to obtain extract liquid; filtering the extract liquid to obtain filter liquid; hydrolyzing the filter liquid by the aid of acid; concentrating the filter liquid to obtain concentrated liquid; precipitating the concentrated liquid to obtain precipitates; redissolving the precipitates by the aid of ethyl alcohol; adding anhydrous sodium carbonate and zinc salt into the precipitates; carrying out reflux reaction to obtain a product; regulating the pH (potential of hydrogen) of the product until the pH of the product reaches 8-10; allowing the product to stand still and precipitating the product; washing the product; drying the product under vacuum conditions to obtain the tea seed flavonoid aglycone and tea sapogenin zinc coordination complex. The tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, the method and the application have the advantages that obvious effects of protecting nerve cells and preventing neurodegeneration can be realized by the tea seed flavonoid aglycone and tea sapogenin zinc coordination complex, and the tea seed flavonoid aglycone and tea sapogenin zinc coordination complex can be applied and developed as a novel medicine for resisting neurodegeneration diseases.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a tea seed flavonoid aglycone and theasapogenin zinc complex with neuroprotective effect and a preparation method thereof, as well as its application as an anti-neurodegenerative disease drug. Background technique [0002] Many components of traditional Chinese medicine have neuroprotective effects, typical flavonoids and saponins. The anti-aging effect of traditional Chinese medicine is the synergistic effect of various components. However, they are often separated and purified in research, which will inevitably reduce the effect. [0003] Tea seeds contain 2-3% flavonoids and 12-14% saponins. The commonly used separation methods of flavonoids and saponins include extraction method, resin method, chromatography, etc. After separation and purification, flavonoids and tea saponins can be obtained respectively. However, the neuroprotective effect of the isolated product is not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07F3/06A61P25/00A61P25/28
CPCC07F3/003C07J63/008
Inventor 叶勇杨谦
Owner SOUTH CHINA UNIV OF TECH
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