A kind of phosphorus-based biochar material and its preparation and application
A biochar and phosphorus-based technology, applied in the restoration of polluted soil, etc., can solve the problems of difficult quantification and easy loss of n-HAP, and achieve the effect of facilitating large-scale production, clean and sustainable process, and stable performance
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Embodiment 1
[0038] To prepare the phosphorus-based biochar material for repairing cadmium-contaminated soil of the present invention:
[0039] (1) Use PDA medium to activate and expand Aspergillus niger (Aspergillus niger), purchased from China Type Culture Collection, strain number CCTCC AF 91006;
[0040] (2) 1.0g of hydroxyapatite nanoparticles (particle size 97%) was added to 200mL of liquid PDA culture medium, and ultrasonic dispersion with a power of 100 watts was used for 2 hours;
[0041] (3) Put the PDA medium containing nano-hydroxyapatite into a high-temperature autoclave, set 115°C, and sterilize for 25 minutes. After high temperature and high pressure sterilization, the culture medium is transferred to the ultra-clean aseptic operation table, and the ultraviolet light is turned on for 60 minutes;
[0042] (4) In an ultra-clean aseptic operating table, inoculate the activated mycelium in step (1) into the sterilized medium of step (3), the inoculation volume is 2 mL; the mass concentr...
Embodiment 2
[0049] The soil was taken from low-polluted paddy soil around a lead-zinc tailings pond. After removing gravel and broken roots, the test soil is air-dried naturally, mixed evenly by quarter method, and crushed through an 18-mesh nylon sieve. The soil pH is 6.58, which is weakly acidic. The content of available Cd in the soil is 7.01 mg / kg.
[0050] Separately add 0.2g of the phosphorus-based biochar material in Example 1 (containing the amount of n-HAP equal to the amount of pure n-HAP weighed) and pure n-HAP into 5.0g of low-polluting soil, mix well, and add 10mL After fixation for 7 days, use 25mL DTPA to extract the effective state of cadmium in the soil. After testing, the available cadmium content of low-polluted soil before and after the phosphorus-based biochar material or pure n-HAP treatment is shown in Table 1.
[0051] Table 1 Effective Cd content before and after treatment in low-polluted soil
[0052]
Embodiment 3
[0054] The soil was taken from moderately polluted paddy soil around a lead-zinc tailings pond. After removing gravel and broken roots, the test soil is air-dried naturally, mixed evenly in quarters, and crushed through an 18-mesh nylon sieve. The soil pH is 6.52, which is weakly acidic. The content of available Cd in the soil is 36.8mg / kg.
[0055] Add 0.2g of the phosphorus-based biochar material in Example 1 (the amount of n-HAP is equal to the amount of pure n-HAP weighed) and pure n-HAP into 5.0g of contaminated soil, mix well, and add 10mL After fixation for 7 days, use 25mL DTPA to extract the effective state of cadmium in the soil. After testing, the effective content of cadmium in the moderately polluted soil before and after treatment with phosphorus-based biochar material or pure n-HAP is shown in Table 2.
[0056] The content of available Cd in the contaminated soil before and after treatment in Table 2
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