Testing method for transgenic ingredients in soybean oil
A technology of genetically modified ingredients and detection methods, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve problems such as failure, and achieve accurate and reliable results
Inactive Publication Date: 2016-05-25
JIANGHAN UNIVERSITY
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Problems solved by technology
[0005] There are many kinds of genetically modified ingredients, and the existing technology can only detect one or a few of them at a time. Therefore, it is necessary to test the possible multiple genetically modified ingredients one by one before they can be determined as "non-genetically
Method used
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Embodiment
[0029] The biolaphos resistance (Bar) gene was introduced into the Dongnong 42 soybean variety by the Agrobacterium-mediated method, and after selfing and purification for 3 generations, the transformed Dongnong 42 soybean was obtained and processed into soybean oil as the present implementation sample to be tested. Among them, Dongnong 42 soybean is a transgenic soybean recipient variety, which was bred by the Soybean Research Institute of Northeast Agricultural University. The Dongnong 42 soybean seeds used in this example were preserved and propagated by Jianghan University, and the transformed Dongnong 42 soybean was pressed. Get soybean oil.
[0030] The method for detecting the genetically modified components of the plant variety to be tested, the specific steps are as follows:
[0031] Step 1. Determine the transgenic components to be detected in the samples to be tested, the endogenous standard genes in the samples to be tested, and the exogenous standard genes in the...
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The invention discloses a testing method for transgenic ingredients in soybean oil and belongs to the technical field of biology. The method comprises the steps that the transgenic ingredients, endogenous standard genes and exogenous standard genes are determined; multiple amplification primers are prepared; sampling and mixing are carried out to obtain a mixed sample; a genome of the mixed sample is extracted, and the exogenous standard genes are added to obtain mixed nucleic acid; a high-throughput sequencing library is built; high-throughput sequencing is performed on the high-throughput sequencing library to obtain a sequencing segment set; the number of sequencing segments of the transgenic ingredients in the sample to be tested, the number of sequencing segments of the endogenous standard genes and the number of sequencing segments of the exogenous standard genes are obtained; according to the number of the sequencing segments of the exogenous standard genes, whether an experiment is successful or not is judged, and the content of the transgenic ingredients in the sample to be tested is calculated; according to the content of the transgenic ingredients , whether the sample to be tested contains the transgenic ingredients or not is judged. Through the method, any and multiple transgenic ingredients in the sample to be tested can be tested quantitatively at the same time.
Description
technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting genetically modified components of soybean oil. Background technique [0002] Soybean is the main food crop in my country. The genetically modified technology of soybean and its products has matured. The sales of genetically modified soybean products need strict approval. Therefore, the detection of genetically modified ingredients in soybean oil is particularly important. [0003] The existing genetically modified component detection technology mainly detects nucleic acid or protein. Among them, the main process of the nucleic acid-based detection method is: extract the nucleic acid in the sample to be tested, and use PCR (Polymerase Chain Reaction, polymerase chain reaction) method to amplify the transgene in the sample. Components, using real-time quantitative, agarose electrophoresis or chip technology to detect the presence of genetically modified c...
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Login to View More IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6895C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2535/122C12Q2545/101
Inventor 陈利红彭海张静陈斌周俊飞
Owner JIANGHAN UNIVERSITY