Direct detection of five chemical pollutants in propolis by electrospray extraction ionization mass spectrometry

Abstract

The invention belongs to the technical field of detection and discloses a method for directly detecting five kinds of chemical pollutants in propolis by electrospray extraction ionization mass spectrometry. The invention comprises the following steps: 1) preparation of standard solution; 2) preparation of propolis tincture; 3) preparation of standard propolis tincture; 4) electrospray extraction ionization mass spectrometry detection; 5) qualitative analysis; 6) quantitative analysis. The detection method of the invention has less sample consumption, fast analysis speed, and can realize high throughput and on-line detection. Direct analysis and detection of common propolis chemical pollutants without complicated sample pretreatment, and the detection time of a single sample is less than 1min. Non-toxic and non-polluting to products, operators and working environment; high sensitivity. The invention does not require complicated pretreatment of the sample to be tested, can withstand the influence of the complex matrix of propolis, realizes direct and rapid detection of chemical pollutants in propolis, and improves the defects of cumbersome sample pretreatment and long analysis time in the existing analysis technology.

Description

technical field [0001] The invention relates to a method for detecting chemical pollutants in propolis, in particular to a method for directly detecting five kinds of chemical pollutants in propolis by electrospray extraction ionization mass spectrometry. Background technique [0002] Propolis is a resin collected by bees from poplar, betula, willow, oak, horse chestnut and coniferous plants, and mixed with beeswax by bees to form a viscous substance through glandular secretions. Propolis is an effective substance used by bees to maintain the health of the whole group. A colony of 50,000 to 60,000 bees can only produce 70 to 100g of propolis a year, so it is called "purple gold". Due to its diverse biological and pharmacological activities, propolis has properties such as antibacterial, antiviral, antioxidative, anticancer, beautifying and promoting cell regeneration, and is widely used in medicine, cosmetics and food industries. [0003] The residue of chemical pollutants ...

AI Technical Summary

Problems solved by technology

At present, the detection of chemical pollutants in propolis mainly includes high performance liquid chromatography (HPLC), gas chromatography (GC), high performance liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), etc. , they have high sensitivity, but the complex sample pretreatment takes a long time and cannot meet the needs of rapid and high-throughput detection

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