Tissue rapid propagation seedling cultivation method for manglietia fadouensis

A technology for rapid propagation of seedlings and tissues, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of lack of seedling cultivation measures, rapid propagation technology and management methods, difficulty in seed collection, and restrictions on the development and utilization of magnanima , to achieve the effect of high induction rate, strong rooted seedlings and short cultivation period

Active Publication Date: 2016-06-01
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of plants that have unique characteristics such as rapid response time, increased yield compared to existing methods or techniques like soil improvement programs. It also includes efficient ways to grow mangliese grasses into these types of crops through controlled conditions during their growing process. Overall, this new method improves crop production efficiency while promoting biodiversity within specific areas.

Problems solved by technology

This patented describes two different types of manglia plants: Mussillosylum Linn., Prunella Magniotis Thermopsis Benth, and Pteridium Spirilaginodes Boissimus Longitudinal Graftsmith). These plant varieties exhibit unique properties that make them ideal for use in various applications such as environmental cleanup, landscaping, vegetable production, biological control agents, medicines, and pesticides. They require specific conditions during their cultivating process due to factors like weather changes, soil erosion, water quality issues, and nutrients supply levels.

Method used

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  • Tissue rapid propagation seedling cultivation method for manglietia fadouensis
  • Tissue rapid propagation seedling cultivation method for manglietia fadouensis
  • Tissue rapid propagation seedling cultivation method for manglietia fadouensis

Examples

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Effect test

Embodiment 1

[0038] A method for rapid propagation of Manglietia fasciatus tissue in tissues includes the following steps:

[0039] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.

[0040] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are scrubbed clean with sterile water on the ultra-clean workbench, and then disinfected with 75% alcohol by volume. The immersion time is 20s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add 0.1% mercury liter solution with mass fraction, soak for 3 minutes, and shake continuously during this period. After the mercury liter solution is poured out, rinse with sterile water for 5-6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are pla...

Embodiment 2

[0047] A method for rapid propagation of Manglietia fasciatus tissue in tissues includes the following steps:

[0048] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.

[0049] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are scrubbed clean with sterile water on the ultra-clean workbench, and then disinfected with 70% alcohol by volume. The soaking time is 30s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add a 0.08% mercury liter solution with mass fraction, soak for 8 minutes, and continuously shake during the period. After discarding the mercury liter solution, rinse with sterile water for 5 to 6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are pla...

Embodiment 3

[0056] A method for rapid propagation of Manglietia fasciatus tissue by tissues includes the following steps:

[0057] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.

[0058] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are wiped clean with sterile water on the ultra-clean workbench, and then disinfected with 75% alcohol by volume. The immersion time is 30s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add 0.05% mercury liter solution with mass fraction, soak for 10 minutes, and shake continuously during the period. After the mercury liter solution is poured out, rinse with sterile water for 5-6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are place...

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Abstract

The invention discloses a tissue rapid propagation seedling cultivation method for manglietia fadouensis, and belongs to the technical field of plant tissue culture. An ML culture medium is set, different hormone components and concentration levels proportion are optimized, seeds are used as explants, complete plants are formed through axillary bud induction, axillary bud proliferation and rooting culture, and after the plants are transplanted to a matrix, robust nursery stock which grows neatly and uniform in appearance is obtained. Axillary bud induction is high in speed, and the inductivity is high; the proliferation coefficient is large, clustered buds are thick and strong, and elongation growth is high in speed; the rooting rate is large, root systems are developed well, rooted seedlings are thick and strong, and the transplant survival rate is large. An effectively path is provided for tissue culture seedling culture of manglietia fadouensis, manglietia fadouensis nursery stock seedlings can be obtained on a large scale through large-scale seedling culture, technical support will be provided for asexual popularization of manglietia fadouensis and even manglietia fordiana plants, and the tissue rapid propagation seedling cultivation method has wide application prospects and is of great significance in landscaping and ecological civilization construction in Guangdong and even the South China.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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