Tissue rapid propagation seedling cultivation method for manglietia fadouensis
A technology for rapid propagation of seedlings and tissues, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of lack of seedling cultivation measures, rapid propagation technology and management methods, difficulty in seed collection, and restrictions on the development and utilization of magnanima , to achieve the effect of high induction rate, strong rooted seedlings and short cultivation period
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Embodiment 1
[0038] A method for rapid propagation of Manglietia fasciatus tissue in tissues includes the following steps:
[0039] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.
[0040] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are scrubbed clean with sterile water on the ultra-clean workbench, and then disinfected with 75% alcohol by volume. The immersion time is 20s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add 0.1% mercury liter solution with mass fraction, soak for 3 minutes, and shake continuously during this period. After the mercury liter solution is poured out, rinse with sterile water for 5-6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are pla...
Embodiment 2
[0047] A method for rapid propagation of Manglietia fasciatus tissue in tissues includes the following steps:
[0048] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.
[0049] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are scrubbed clean with sterile water on the ultra-clean workbench, and then disinfected with 70% alcohol by volume. The soaking time is 30s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add a 0.08% mercury liter solution with mass fraction, soak for 8 minutes, and continuously shake during the period. After discarding the mercury liter solution, rinse with sterile water for 5 to 6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are pla...
Embodiment 3
[0056] A method for rapid propagation of Manglietia fasciatus tissue by tissues includes the following steps:
[0057] (1) Explant collection: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, with an altitude of about 1700m, a tree age of about 70 years, and a tree height of about 30m.
[0058] (2) Surface disinfection of explants: the Manglietia fasciatus seeds are wiped clean with sterile water on the ultra-clean workbench, and then disinfected with 75% alcohol by volume. The immersion time is 30s. After the alcohol is poured out, it will be sterile. Rinse with water for 1 to 2 times, then add 0.05% mercury liter solution with mass fraction, soak for 10 minutes, and shake continuously during the period. After the mercury liter solution is poured out, rinse with sterile water for 5-6 times to obtain sterile seeds. The sterile filter paper absorbs water droplets on the surface of the sterile material, and the sterile seeds are place...
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