Nucleic acid processing device, nucleic acid processing equipment and kit based on magnetic bead method

Abstract

The invention discloses a nucleic acid processing device based on a magnetic bead method, which includes a first pipeline, a second pipeline and a reaction chamber connecting the first pipeline and the second pipeline. Both the first pipeline and the second pipeline include a larger end and a larger end. The small end and the larger end are used for external sampling and sampling. The reaction chamber has a first connection end, a reaction chamber main body, and a second connection end connected in sequence. The first connection end is connected to the smaller end of the first pipeline, and the second The connecting end is connected to the smaller end of the second pipeline, and the width of the first connecting end and the width of the second connecting end are both smaller than the width of the main body of the reaction chamber. In the nucleic acid processing device based on the magnetic bead method, one end of each pipeline is large and the other end is small. The larger end is used for sampling and adding samples. During sampling and adding samples, the gas is not easy to overflow into the reaction chamber, which improves the reaction efficiency. The reaction chamber designed with small ends and a large middle can reduce the direct contact area of ​​the liquid phases of two adjacent pipes and prolong the miscibility time. The invention also discloses a kit and a nucleic acid processing device.

Description

technical field [0001] The invention relates to the technical field of chemical substance processing, in particular to a nucleic acid processing device based on a magnetic bead method, nucleic acid processing equipment and a kit. Background technique [0002] As we all know, nucleic acid is an important carrier of biological information and the main object of molecular biology research. The quality of nucleic acid samples directly affects the results of experiments. Therefore, how to improve the extraction rate and purity of nucleic acid samples has always been one of the important research directions for researchers. [0003] Nucleic acid (DNA and RNA) extraction involves cell disruption, nucleic acid purification and isolation steps. Cell rupture can be achieved by physical and mechanical disruption and chemical enzymatic hydrolysis. There are also various methods for nucleic acid purification and separation: for example, there is a concentrated salt method that uses dif...

AI Technical Summary

Problems solved by technology

Although there are devices and equipment used to solve the above problems in the prior art, the purity of the nucleic acid molecules extracted by these devices is still not high, and there are still impurities in the nucleic acid molecules, and the operation of using these devices to extract nucleic acid molecules is complicated and time-consuming. , high cost

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