Protein molecule active separation and representation combination system

Abstract

The invention discloses a protein molecule active separation and representation combination system, comprising an asymmetric flow field flow separometer and an electrospray ionization mass spectrometer, wherein a sample outlet of the asymmetric flow field flow separometer is connected with a sample inlet of the electrospray mass spectrometer. In this way, the asymmetric flow field flow separometer is used as a separation instrument in the system, compared with a common conventional protein separation method, the separation condition of the asymmetric flow field flow separometer is mild, the shear force action of a separation medium to protein components is avoided, the stereochemical structure of protein molecules can be kept in a separation and purification process, the separated and purified protein molecules have the stability and activity of the original structure, a separated protein sample does not need to be subjected to after treatment, operation time can be shortened, and operation procedures are simplified; the electrospray mass spectrometer is used as a universal and sensitive detection instrument for performing component and structure analysis on the protein components separated by the asymmetric flow field flow separometer, and the representing is quick and accurate.

Description

technical field [0001] The present application relates to the technical field of protein separation, in particular to a combined system for separation and characterization of protein molecule activity. Background technique [0002] The rapid development of proteomics requires research on advanced separation and characterization systems. The separation technology in this system should have the advantages of rapid separation, high selectivity and wide application range, while not destroying the activity and stability of the target protein during the separation and purification process; and the characterization technology in this system should have operational Simple, high characterization sensitivity and other advantages. [0003] In the commonly used traditional protein separation methods, electrophoresis technology needs to add surfactant, ion exchange chromatography method needs to add salt buffer solution, liquid chromatography method needs to add organic solution, and it...

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Problems solved by technology

[0003] In the commonly used traditional protein separation methods, electrophoresis technology needs to add surfactant, ion exchange chromatography method needs to add salt buffer solution, liquid chromatography method needs to add organic solution, and it is carried out in a stationary phase chromatographic column, all of which will destroy the stability of protein molecules. Three-dimensional structure, and easy to induce the splitting of protein components

[0004] The commonly used traditional protein characterization method, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) technology needs to separate the separated protein samples for post-processing, which takes a long time and is inconvenient to operate

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