Method for culturing three-way cross hybrid of melons by single-female lines

Abstract

The invention relates to a method for culturing three-way cross hybrid of melons by single-female lines, comprising the following steps: selecting parents: (1) breeding single female inbred lines with good traits as a single cross hybrid female parent; breeding two male all-flower plant inbred lines with good traits, wherein one of the two male all-flower plant inbred lines is taken as a single cross hybrid male parent, and the other one is taken as a three-way cross hybrid male parent; (2) culturing single cross hybrid: carrying out hybridization on the single cross hybrid female parent and the single cross hybrid male parent to prepare a single-female single cross hybrid, wherein during hybridization, paper hats sleeve the single cross hybrid female parent and the single cross hybrid male parent at the time of 1 day before flowering of female flowers of the single cross hybrid female parent and male flowers of the single cross hybrid male parent for isolation; (3) culturing three-way cross hybrid: preparing the three-way cross hybrid by taking the single-female single cross hybrid as the female parent and male parent of the three-way cross hybrid through a single cross hybrid hybridization method. According to the method, the female parent which is hybridized for twice has single femina, so that the advantage that bud stripping and castration are not needed to be carried out in the two-time hybridization is exerted, a seed production process is simplified, the seed cost is lowered, and the seed purity is improved; furthermore, the heterosis of the melons is fully exerted, and the valuable genes of three different parent materials are combined, so that an excellent variety with prominent economic traits can be obtained.

Description

technical field [0001] The invention relates to a method for hybrid seed production of melons, in particular to a method for cultivating three-cross melons by using a single female line. Background technique [0002] Melon has significant heterosis. In recent years, the hybrid seed production of melon is mainly two-line hybrid, and there is also a small amount of three-cross seed production. Among them, the two-line hybridization method is simple and practical, but has the following defects: (1) when the parents are closely related and the combining ability is low, the seed production yield is low and the heterosis is not obvious enough; (2) the parents are easy to lose. Although the three-cross seed production is beneficial to aggregate the excellent traits of multiple seed-producing parents and better exert the heterosis of muskmelon, the process of hybrid seed production is more complicated than the single-cross seed production procedure, especially when the two crosses a...

AI Technical Summary

Problems solved by technology

Among them, the two-line hybridization method is simple and practical, but it has the following defects: (1) when the parental relationship is close and the combining ability is low, the seed production yield is low and the heterosis is not obvious enough; (2) the parents are easy to lose

Although the three-cross seed production is beneficial to aggregate the excellent traits of multiple seed-producing parents and better exert the heterosis of muskmelon, the process of hybrid seed production is more complicated than the single-cross seed production procedure, especially when the two crosses are performed, the fruit setting and flowering of the female parent will be affected. The requirements for peeling and emasculation of buds are extremely strict, otherwise it is easy to cause false hybrids, resulting in low seed purity and scrapping

So far, no single female line has been used in three-cross melon seed production

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