9,9'-(3,3'-Dihydroxy-4,4'-diphenylether)bifluorone reagent and preparation method and application thereof

A technology of difluorone and diphenyl ether-based diformaldehyde, applied in chemical instruments and methods, luminescent materials, chemiluminescence/bioluminescence, etc., can solve the problems of unsatisfactory sensitivity and selectivity, and achieve stable properties and fast speed , the effect of simple preparation method

Inactive Publication Date: 2016-07-06
SHANXI DATONG UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a 9,9'- (3,3'-dihydroxy-4,4'-dipheny

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 9,9'-(3,3'-Dihydroxy-4,4'-diphenylether)bifluorone reagent and preparation method and application thereof
  • 9,9'-(3,3'-Dihydroxy-4,4'-diphenylether)bifluorone reagent and preparation method and application thereof
  • 9,9'-(3,3'-Dihydroxy-4,4'-diphenylether)bifluorone reagent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0020] Example 1: A 9,9'-(3,3'-dihydroxy-4,4'-diphenyl ether group) difluorone reagent, referred to as 9,9'-DHDPDF, its molecular structural formula is:

[0021] .

[0022] The preparation method of the 9,9'-(3,3'-dihydroxy-4,4'-diphenyl ether group) bisfluorone reagent comprises the following steps:

[0023] (1) Synthesis of trimerol triacetate: add 28mL acetic anhydride and 1mL concentrated H to a 100mL beaker 2 SO 4 Stir and mix, add 11g of p-benzoquinone under vigorous stirring, react at 40°C-50°C for 1.5h, pour it into 100mL of cold water under stirring, a white precipitate is precipitated, 22g of crude product is obtained by suction filtration, and the pure product is obtained by recrystallization from ethanol Phenol triacetate 20g;

[0024] (2) Preparation of 3,3'-dihydroxy-4,4'-diphenyl ether dicarbaldehyde: Add 10.1g (50mmol) 3,3'-dihydroxydiphenyl into a 250mL flask equipped with a reflux condenser Ether, 120mL of absolute ethanol, 28g (0.7mol) of sodium hydrox...

experiment example １

[0026] Experimental example 1: 9,9'-(3,3'-dihydroxy-4,4'-diphenyl ether group) difluorone on Co 2+ photometric analysis.

[0027] Accurately pipette ≤20μg of Co 2+ In a 25mL volumetric flask, add 2.0mL borax-sodium hydroxide buffer solution with pH 9.0, 10g / L cetylpyridinium bromide (CPB) microemulsion (CPB: n-butanol: n-heptane: water) =1.0:1.0:1.0:97) 3.0mL, 0.2g / L9,9'-DHDPDF1.0mL, then dilute to the mark with double distilled water, shake well and let stand for 10min, use a 1cm cuvette, at 660nm, The absorbance of the complex was measured with the reagent blank as a reference.

[0028] Sample analysis: according to the literature [Journal of Pharmaceutical Analysis, 1997, Vol. 77, No. 4, 261-264. Wei Qin, Du Bin, Zhang Xuzhen], pipette vitamin B 12 Put 1.0mL of the sample in a 50mL beaker, add 8mol / L nitric acid for nitrification, add 5mL of 2% hydrogen peroxide and steam until nearly dry, add 3mL of 1:1 hydrochloric acid and appropriate amount of water to dissolve the s...

experiment example ２

[0035] Experimental example 2: Determination of nickel by 9,9'-(3,3'-dihydroxy-4,4'-diphenyl ether group) bisfluorone fluorescence quenching method.

[0036] Accurately weigh ≤0.005μg nickel in a 25ml volumetric flask, add 2mL of 3% Tritonx-100 aqueous solution, 3.0mL of borax-hydrochloric acid buffer solution with pH9.4, 2×10 -5 mol / L 9,9'-DHDPDF ethanol solution 2.0mL, dilute to volume with water, let stand for 10min. Using a 1cm quartz cuvette, at the excitation wavelength (λ ex )465nm, emission wavelength (λ em ) at 586nm to measure the fluorescence intensity F of the complex, and simultaneously measure the fluorescence absorbance F of the reagent blank under this condition, and calculate the fluorescence quenching value ΔF (ΔF=F 0 -F).

[0037] Sample determination: Weigh 1.0000g of solid sample, carry out microwave digestion to the sample according to the literature [Food Science, 2011, Vol. Volume 2, pp. 158-160, Wei Qin] separated and enriched nickel, and the resul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of phenylfluorone compounds and provides a 9,9'-(3,3'-dihydroxy-4,4'-diphenylether)bifluorone reagent and a preparation method and application thereof to solve the problem that an existing phenylfluorone compound having non-ideal sensitivity and selectivity is unable to serve as a fluorescent reagent in the fluorescent detection of metal ions.Two pyranoid ring structural units are introduced in molecules, the quantity of metal ion coordination sites is increased by one time, the ability of the reagent to coordinate with metal ions is enhanced, and the fluorescent reagent good in sensitivity and selectivity is obtained.The preparation method is simple, the cost is low, properties are stable, a detection limit in nickel determination by fluorophotometric quenching process in a basic medium in the presence of a surfactant is lower that of other reagent quenching processes, cobalt determination by spectrophotometry is higher than other fluorone reagent processes in sensitivity, and a detection limit in cobalt determination by discoloring spectrophotometry is lower than that of a process using other fluorone reagents.This reagent has high detection speed for nickel and cobalt ions and is good in selectivity, high in sensitivity and high in complex stability.

Description

technical field [0001] The invention belongs to the technical field of phenylfluorone compounds, and specifically relates to a 9,9'-(3,3'-dihydroxy-4,4'-diphenyl ether group) difluorone reagent and its preparation method and application . Background technique [0002] Fluorone reagents refer to a class of triphenylmethane derivatives containing 2,3,7-trihydroxy-6-keto-pyran ring structure in the molecule. The compound formed when the 9-position hydrogen atom is replaced by phenyl is also known as phenylfluorone (see structure figure 1 ). Phenylfluorone is mostly orange or red crystals, hardly soluble in water and cold ethanol, but easily soluble in acidified ethanol. Molecules of such compounds are rigid, planar conjugation and large Bond structure, easy to absorb ultraviolet-visible light and excited to produce fluorescence. Its color reaction functional group is the o-dihydroxy group or o-hydroxyquinone group at both ends of the molecule. Oxygen atoms in hydroxyl an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09K11/06C07D311/82G01N21/76
CPCC07D311/82C09K11/06C09K2211/1088G01N21/76
Inventor 刘慧君樊月琴赵强马琦樊志会宋金萍
Owner SHANXI DATONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap