Unlock instant, AI-driven research and patent intelligence for your innovation.
A culture medium and its method for cultivating ornamental pineapple seedlings
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for ornamental pineapple and culture medium, applied in the field of biological tissue culture, can solve the problems of manpower and material resources, and achieve the effects of reducing workload, reducing mutation rate, and saving production costs
Inactive Publication Date: 2017-11-17
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Ornamental pineapple tissue culture seedlings are generally subcultured every three months, which requires manpower and material resources. At the same time, because the culture materials are stimulated by exogenous hormones for a long time, the probability of mutation will increase with the increase of subculture times.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] A culture medium, comprising: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L.
[0021] A method for cultivating ornamental pineapple seedlings using the described culture medium, comprising the following steps:
[0022] (1) Preparation of delayed subculture medium: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L, the medium was adjusted to pH 5.8 before sterilization;
[0023] (2) Insert the pineapple tissue culture seedlings into the delayed medium, and preserve under the culture conditions of temperature 25±3°C, light 2000Lx, and light time 12h / d;
[0024] (3) When it is found that the delayed medium is close to dryness or needs to be re-amplified, it can be directly transferred to a new medium.
Embodiment 2
[0026] A culture medium, comprising: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L.
[0027] A method for cultivating ornamental pineapple seedlings using the described culture medium, comprising the following steps:
[0028] (1) Preparation of delayed subculture medium: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L, the medium was adjusted to pH6.0 before sterilization;
[0029] (2) Insert the pineapple tissue culture seedlings into the delayed medium, and preserve under the culture conditions of temperature 25±3°C, light 2000Lx, and light time 14h / d;
[0030] (3) When it is found that the delayed medium is close to dryness or needs to be re-amplified, it can be directly transferred to a new medium.
Embodiment 3
[0032] A culture medium, comprising: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L.
[0033] A method for cultivating ornamental pineapple seedlings using the described culture medium, comprising the following steps:
[0034] (1) Preparation of delayed subculture medium: MS+NAA0.01mg / L+sucrose 25g / L+agar 4.0g / L+potato juice 300g / L, the medium was adjusted to pH5.9 before sterilization;
[0035] (2) Insert the pineapple tissue culture seedlings into the delayed medium, and preserve under the culture conditions of temperature 25±3°C, light 2000Lx, and light time 13h / d;
[0036] (3) When it is found that the delayed medium is close to dryness or needs to be re-multiplied, it can be directly transferred to a new medium.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention belongs to the technical field of biological tissue culture and particularly relates to a culture medium and a method for culturing ornamental bromeliads subculture plantlets by using the same. The culture medium is prepared from 0.01 mg / L of MS+NAA, 25 g / L of saccharose, 4.0 g / L of agar and 300 g / L of potato juice. The method for culturing the ornamental bromeliads subculture plantlets by using the culture medium comprises steps as follows: (1) preparation of a delaying subculture culture medium: the culture medium is prepared from 0.01 mg / L of MS+NAA, 25 g / L of the saccharose, 4.0 g / L of the agar and 300 g / L of the potato juice, and the pH is adjusted to range from 5.8 to 6.0 before sterilization of the culture medium; (2) the delaying subculture culture medium is inoculated with bromeliads tissue culture plantlets and stored under culture conditions of the temperature of 25 DEG C plus / minus 3 DEG C, illumination of 2,000 Lx and the illumination time of 12-14 h / d; (3) after the delaying subculture culture medium is found to be close to be dried up or propagation is required to be expanded again, the plantlets are transplanted to a new culture medium directly. With the adoption of the culture medium, the subculture interval of the bromeliads tissue culture plantlets is prolonged to 18-24 months from 3 months, so that the workload of subculture inoculation is greatly decreased, the aberration rate of the tissue culture plantlets is decreased, and the production cost is saved.
Description
technical field [0001] The invention belongs to the technical field of biological tissue culture, in particular to a culture medium and a method for cultivating secondary seedlings of ornamental pineapple. Background technique [0002] In vitro preservation of plant germplasm resources is the tissue culture preservation of plant explants in a sterile environment, which can overcome the shortcomings of conventional field preservation methods, does not occupy land, saves storage space, eliminates daily management, and facilitates transportation and communication, etc. . [0003] At present, the germplasm preservation of ornamental pineapple mostly adopts the method of in vitro preservation at home and abroad. However, in the process of tissue culture preservation of germplasm, due to the depletion of nutrients in the medium and evaporation of water, the culture must be transferred in time at regular intervals. Inoculate into fresh medium to ensure normal growth of the culture...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More