CDKN2A gene fragment and application of primers of CDKN2A gene fragment in diagnosing tumors

A gene fragment and tumor diagnosis technology, applied in the field of genes, can solve the problem of undetermined CDKN2A gene fragment, etc., and achieve the effect of wide application, cost reduction, and easy operation.

Inactive Publication Date: 2016-07-20
GUANGDONG MEDICAL UNIV
View PDF9 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, a suitable CDKN2A gene fragment has not yet been identified, which can be used as a biomarker for tumor diagnosis, efficacy monitoring, and prognosis by measuring its methylation level

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CDKN2A gene fragment and application of primers of CDKN2A gene fragment in diagnosing tumors
  • CDKN2A gene fragment and application of primers of CDKN2A gene fragment in diagnosing tumors
  • CDKN2A gene fragment and application of primers of CDKN2A gene fragment in diagnosing tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention will be further illustrated by specific examples of the methylation detection of CDKN2A gene fragments in the blood of tumor patients and normal people.

[0021] 1) Genomic DNA extraction and bisulfite treatment.

[0022] The DNA extraction kit was used to extract the total genomic DNA of the blood sample, and the purity and concentration of the DNA solution were detected by agarose gel electrophoresis and ultraviolet-visible light spectrophotometry. Take a certain amount of genomic DNA with a known concentration and treat it with a bisulfite conversion kit to convert unmethylated cytosine (C) into uracil (U), while methylated cytosine remains constant.

[0023] 2) The following describes the specific design method of PCR primers for CDKN2A gene fragments of the present invention as follows:

[0024] Download the full-length human CDKN2A gene sequence from the nucleic acid sequence database Genbank (http: / / www.ncbi.nlm.nih.gov / genbank / ) (sequence registra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a CDKN2A gene fragment and application of the primers of CDKN2A gene fragment in diagnosing tumors.By designing an upstream and downstream primer pair, the specific CDKN2A gene fragment is determined, the gene fragment shows remarkable high DNA methylation in tumor patients while showing low DNA methylation in normal people, and statistic differences exist between DNA methylation of tumor patients and that in normal people.Occurrence and development of tumors are tracked with the level changes of DNA methylation of the gene fragment, and thus a novel biomarker is provided for diagnosis, treatment effect monitoring and prognosis improvement judgment of tumors.

Description

Technical field [0001] The invention belongs to the field of gene technology, and specifically relates to the application of CDKN2A gene fragments in the fields of tumor clinical diagnosis, curative effect monitoring and prognosis judgment. Background technique [0002] Tumor is a common and frequently-occurring disease. At present, malignant tumors have become one of the most serious diseases that endanger human health. The mortality rate of malignant tumors ranks second only to cardiovascular system diseases, and is gradually increasing, consuming a lot of medical resources. Most tumors are caused by the gradual accumulation and interaction of genetic mutations and epigenetic changes, and their development is slow. If they can be detected at an early stage, surgical resection and radical treatment can be performed. Therefore, early tumor screening is of great significance. Existing tumor screening methods include colonoscopy, stool sDNA examination, serum carcinoembryonic anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68C12N15/11
CPCC07K14/4703C12Q1/6883C12Q2600/158
Inventor 蔡春叶晓霞张立坚黄琼林张俊杰文娟
Owner GUANGDONG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap