A kind of method for inducing adventitious buds of mint leaves and plant regeneration
A bud induction and leaf technology, applied in the biological field, can solve the problem of relatively few systematic studies on the regeneration system of mint leaves, and achieve the effect of optimizing the regeneration system of mint plant tissue culture and simplifying the steps.
Active Publication Date: 2017-12-15
凤阳县经济发展投资有限公司
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[0003] At present, there are many researches on mint, and its medicinal value and basic research, such as the research on the extraction of chemical components and the determination of the content of mint chemical components, have been relatively mature. There are also some related reports on plant biotechnology, but the regenerative system of mint leaves There are relatively few systematic studies
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[0021] Preferred embodiments of the present invention are described below in conjunction with accompanying drawing:
[0022] The test material in the embodiment of the present invention is mint test-tube seedlings with a leaf age of 25-30 days preserved in the horticultural tissue culture room of Anhui Institute of Science and Technology. The culture conditions in the following experiments: culture room temperature (25±1)°C, light intensity 1800-2000lx, light time 12h / d.
[0023] 1) Screening of leaf callus induction and differentiation medium: In an ultra-clean workbench, put the leaves on sterile filter paper and use a scalpel to remove the edge and tip of the leaves, cut into a size of about 0.5cm×0.5cm and insert them indefinitely bud induction medium. The adventitious bud induction medium used in the test was based on MS adding 0.2mg / L NAA as the basic medium, adding different concentrations of TDZ and 6-BA (see Table 1 for each combination), sucrose 30g / L, agar 5.5g / L, ...
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The invention relates to a mint leaf adventitious bud induction and plant regeneration method, and belongs to the technical field of biologics.With mint sterile tube seedling leaves as the material, according to an established mint leaf in-vitro regeneration system, a culture medium of healing tissue induction and differentiation includes MS, 1.4 mg / L TDZ, 0.2 mg / L NAA, 30 g / L cane sugar and 5.5 g / L agar, a culture medium of adventitious bud proliferation includes MS, 0.5 mg / L 6-BA, 0.1 mg / L IAA, 30 g / L cane sugar and 5.5 g / L agar, and a culture medium of adventitious bud rooting includes MS, 0.2 mg / L IBA, 20 g / L cane sugar and 5.5 g / L agar.The inoculation parts are mint leaves without leaf edges or leaf tips, and a certain research foundation is laid for mint genetic transformation by means of the established mint leaf in-vitro regeneration system.
Description
technical field [0001] The invention relates to a method for obtaining regenerated plants of mint leaves by culturing mint leaves, and belongs to the field of biotechnology. Background technique [0002] Mint (Mentha haplocalyx Briq.) is a perennial herb of the genus Mentha (MenthaL.) in the Labiatae family. The aboveground part can be used as medicine after drying, and it is an important Chinese herbal medicine and spice plant in our country. In terms of medicine, peppermint can relieve heat, wake up the stomach and open the spleen, relieve itching and pain, and has high medicinal value. Menthol in peppermint essential oil is an important spice, mainly used in cigarettes, cosmetics, toothpaste, chewing gum, soap, refreshing drinks and medicines. [0003] At present, there are many researches on mint, and its medicinal value and basic research, such as the research on the extraction of chemical components and the determination of the content of mint chemical components, ha...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 舒英杰周玉丽胡能兵张雪平隋益虎王虹
Owner 凤阳县经济发展投资有限公司