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Stenotrophomonas maltophilia producing keratinase and its application

A Stenotrophomonas and keratinase technology, applied in microbial strains and application fields, can solve problems such as difficult direct utilization, difficult biodegradation, energy and environmental pollution, etc., and achieve good stability, high degradation rate, and high keratinase activity Effect

Active Publication Date: 2019-07-19
GUANGDONG WENS DAHUANONG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because its main component, keratin, is a hard protein with strong resistance and difficult to biodegrade, it is difficult to use directly, and traditional physical and chemical processing methods: high temperature and high pressure, acid-base treatment, etc. not only consume a lot of energy but also cause serious problems. environmental pollution problems

Method used

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  • Stenotrophomonas maltophilia producing keratinase and its application
  • Stenotrophomonas maltophilia producing keratinase and its application
  • Stenotrophomonas maltophilia producing keratinase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 is used to illustrate the screening and identification process of bacterial classification:

[0047] 1. Screening method for strains producing keratinase:

[0048] 1) Soil samples were collected from a chicken farm of Guangdong Wens Food Group Co., Ltd.;

[0049] 2) Mix the collected samples in sterile distilled water, take the supernatant after standing, press 10 -6 、10 -7 、10 -8 After performing gradient dilution, spread on the agar plate medium containing milk; then place it in a 37°C incubator and incubate it upside down for 24 hours;

[0050] 3) Select a single colony that can produce a transparent degradation circle on the agar plate medium, inoculate it in a liquid inorganic salt medium containing feathers, and culture it with shaking at a temperature of 37 ° C and 180 rpm for 72 hours to obtain a culture medium for the strain; finally select The culture solution of strains with obvious feather degradation is inoculated into a new feather-contain...

Embodiment 2

[0077] Embodiment 2 is used to illustrate the impact of different pH and different fermentation temperatures on the degradation of feathers by Stenotrophomonas maltophilia:

[0078] 1) Prepare 50 mL of liquid inorganic salt medium with a pH of 4 to 11 according to the gradient;

[0079] 2) Add 0.5 mL of Stenotrophomonas maltophilia and 0.5 g of feathers to each liquid inorganic salt medium prepared in step 1), and ferment for 24 hours under the conditions of rotation speed of 220 rpm and temperature of 37°C , detect the degradation rate of feathers by weight loss method;

[0080] 3) The degradation rate of different pH to feather is as follows: image 3 Shown: the results show that when the pH of the liquid inorganic salt medium=9, the degradation rate of feathers is the highest;

[0081] 4) Take the strain of Stenotrophomonas maltophilia, add it to the liquid inorganic salt medium with pH = 9, at the rotation speed of 220rpm, at the temperature conditions of 27°C, 32°C, 37°...

Embodiment 3

[0084] Embodiment 3 is used to illustrate the impact of the concentration of the substance containing keratin in the liquid inorganic salt medium on the feather degradation rate:

[0085] It is respectively 10g / L (1%), 20g / L (2%), 50g / L (5%) and 100g / L (10%) liquid inorganic salt culture medium that the preparation contains feather concentration, in the inoculum size of bacterial classification 1%, pH=9, temperature is fermentation 24h under the condition of 37 ℃; The degradation rate of feather is as follows Figure 5 Shown: when the content of feather in the liquid inorganic salt medium is 10g / L (1%), the degradation rate of feather is the highest; When feather content is greater than 20g / L (2%), the degradation rate of feather increases with feather content increase and then a rapid decline.

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Abstract

The invention discloses a keratinase-producing Stenotrophomonas maltophilia and its application. The Stenotrophomonas maltophilia was preserved in the China General Microorganism Culture Collection and Management Center on March 7, 2016, with a preservation number It is CGMCC No.12186; the Stenotrophomonas maltophilia that produces keratinase degrades keratin-containing substances, comprising the following steps: 1) preparing a liquid inorganic salt medium; 2) taking Stenotrophomonas maltophilia species and keratin-containing substances are added to the liquid inorganic salt medium prepared in step 1), and the inoculum size of the Stenotrophomonas maltophilia strain in the liquid inorganic salt medium is a, 0.5% ≤a≤5%, ferment under the conditions of rotating speed 150~220rpm and temperature 27~47℃ to degrade keratin-containing substances; Keratin can be efficiently degraded in the medium.

Description

technical field [0001] The invention relates to the field of microbial strains and applications, in particular to Stenotrophomonas maltophilia producing keratinase and its application. Background technique [0002] Feather is an important protein resource, and its protein content is as high as 85-90%. However, because its main component, keratin, is a hard protein with strong resistance and refractory biodegradation, it is difficult to use directly, and the traditional physical and chemical processing methods: high temperature and high pressure, acid-base treatment, etc. not only consume a lot of energy but also cause severe environmental pollution problems. At present, the annual production of feather waste in my country's aquaculture industry has reached more than one million tons. With the continuous development of my country's aquaculture industry, this amount is still growing. It is an inevitable choice to realize high-value transformation and recycling of waste resour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 黄妙容钟楚红任广彩刘传高陈瑞爱叶俊贤
Owner GUANGDONG WENS DAHUANONG BIOTECH
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