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Fluorescent microscopic image three-dimensional reconstruction method and system based on compound regularization technology

A microscopic image and three-dimensional reconstruction technology, applied in the field of image processing and computer vision, can solve the problems of poor reconstruction effect and inability to preserve the edge and texture information of the image at the same time, so as to suppress the ladder effect, good effectiveness and feasibility High effectiveness, effectiveness and feasibility

Inactive Publication Date: 2016-08-10
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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Problems solved by technology

However, these current technologies cannot preserve the edge and texture information of the image at the same time, and suppress the step effect of the smooth area, which makes the reconstruction effect poor.

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  • Fluorescent microscopic image three-dimensional reconstruction method and system based on compound regularization technology
  • Fluorescent microscopic image three-dimensional reconstruction method and system based on compound regularization technology
  • Fluorescent microscopic image three-dimensional reconstruction method and system based on compound regularization technology

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Embodiment Construction

[0050] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0051] The method and system for three-dimensional reconstruction of fluorescence microscopic images based on compound regularization technology according to the embodiments of the present invention will be described below with reference to the accompanying drawings.

[0052] figure 1 is a flowchart of a method for three-dimensional reconstruction of fluorescence microscopic images based on compound regularization technology according to an embodiment of the present invention. like figure 1 As shown, according to an embodiment of the p...

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Abstract

The invention provides a fluorescent microscopic image three-dimensional reconstruction method and system based on compound regularization technology. The method comprises the steps of acquiring a fluorescent microscopic image, and establishing a degeneration model for the fluorescent microscopic image; carrying out compound regularization constraints of generalized total variation and shearlet transform for the degeneration model, to obtain an object function of fluorescent microscopic image reconstruction; obtaining an unconstrained object function equivalent to the object function according to the theory of separation of variable; and according to the unconstrained object function, calculating a reconstructed image of the fluorescent microscopic image based on a split Bregman iterative algorithm. The method of the invention can obtain an optimal reconstructed original effect by reasonable selection of parameters, and is high in effectiveness and feasibility.

Description

technical field [0001] The invention relates to the technical fields of image processing and computer vision, in particular to a method and system for three-dimensional reconstruction of fluorescence microscopic images based on composite regularization technology. Background technique [0002] Optical microscopy has long been a powerful tool for observing the microstructure of biological samples. However, sometimes it is difficult to distinguish small differences in deep cells or tissue properties. Combined with fluorescent labeling technologies such as fluorescent probes and green fluorescent protein, users can observe more and clearer sample structure information. With the help of sample slice image sequences and three-dimensional (threedimensional / 3D) reconstruction technology, the 3D three-dimensional structure of the sample can also be obtained , providing a means for studying the dynamic change process and rules of molecules and cells, and the relationship between bra...

Claims

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Application Information

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IPC IPC(8): G06T17/00
CPCG06T17/00
Inventor 王瑜张长春肖洪兵
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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