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Preparation method of phorate pesticide hapten and application of phorate pesticide hapten

A hapten, hydroxylated phorate technology, applied in the field of immunoassay, can solve problems such as not being well solved, and achieve the effect of good specificity and high titer

Inactive Publication Date: 2016-08-24
XUZHOU NORMAL UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that the key issue of hapten design in this field has not been well resolved

Method used

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  • Preparation method of phorate pesticide hapten and application of phorate pesticide hapten
  • Preparation method of phorate pesticide hapten and application of phorate pesticide hapten
  • Preparation method of phorate pesticide hapten and application of phorate pesticide hapten

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Embodiment approach 1

[0026] The preparation method of embodiment 1 hapten B (I)

[0027] Put P in the 50mL reaction bottle 5 S 2 11.1g, then dropwise into absolute ethanol 9.66g, the reactant becomes a suspension from a slurry, the temperature gradually rises, and emits H 2 S. When the temperature rises to about 80°C, control the rate of addition, keep the temperature in a water bath, and stir for 1 hour, and stop the reaction until the powder is completely dissolved. Distilled water was extracted three times, and the organic phase was discarded to obtain sulfide in the aqueous phase. Put the sulfide in the reaction bottle, add 10g of 30% formaldehyde solution dropwise at room temperature, and after magnetically stirring for 20min, add 7.9g of 2-mercaptoethanol to control 60°C and react for 5 hours. After concentrating, pass through a silica gel chromatography column to collect a sample and complete purification to obtain 12 g, with a yield of 31%. Weigh 0.552g (2mmol) of it, dissolve it in 1...

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Abstract

The invention discloses a method for increasing antigenicity of phorate pesticide. Phorate is 0,0-diethyl-S-[(ethylsuleenyl)methyl]phosphorodithioate, and the CAS number is 298-02-2; the hapten is the product obtained by combining hydroxide of the phorate pesticide and an acylate-chlorinated 1,8-naphthyridine compound. A series of hapten is provided for the micromolecular organophosphor phorate, different acylate-chlorinated 1,8-naphthyridine compounds are introduced into the hapten on the basis of phorate oxygen atoms, therefore, the hapten can be connected with carrier protein more conveniently, the antigenicity of the phorate pesticide is increased, the preparation success rate of the phorate pesticide hapten is higher, and a technological base is provided for preparation, fast detection and product development of anti-phorate pesticide antibodies.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to an optimization method for preparing phorate pesticide hapten. Background technique [0002] Organophosphorus pesticides are organic compounds containing C-P bonds or C-O-P, C-S-P, C-N-P bonds. There are dozens of official products, such as parathion, omethoate, dichlorvos, malathion, phoxim, phorate, Monocrotophos, methamidophos, trichlorfon, dimethoate, methyl 1605, azinphos, etc. Most organophosphorus pesticides are insoluble in water but soluble in organic solvents, stable under neutral and acidic conditions, not easily hydrolyzed, and easily hydrolyzed and invalidated under alkaline conditions. Organophosphorus pesticides (organophosphate pesticides) form phosphorylated cholinesterase with cholinesterase in the body, and the activity of cholinesterase is inhibited, so that the enzyme cannot catalyze the hydrolysis of acetylcholine, resulting in excessive accumulation of acety...

Claims

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Application Information

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IPC IPC(8): C07F9/6561G01N33/53
Inventor 陈才法吴翚孙东旭周生亮宛瑜
Owner XUZHOU NORMAL UNIVERSITY
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