Kit and method for combining and analyzing CD11c+CD11b+DC subgroup and differentiation degree and functions thereof

A combined analysis and kit technology, applied in the field of immunological detection, can solve problems such as extensive analysis schemes and inability to meet the precise analysis requirements of dendritic cells

Inactive Publication Date: 2016-08-31
YEACELL BIOTECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current flow cytometry analysis scheme for dendritic cells is too extensive and...

Method used

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  • Kit and method for combining and analyzing CD11c+CD11b+DC subgroup and differentiation degree and functions thereof
  • Kit and method for combining and analyzing CD11c+CD11b+DC subgroup and differentiation degree and functions thereof
  • Kit and method for combining and analyzing CD11c+CD11b+DC subgroup and differentiation degree and functions thereof

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Embodiment Construction

[0032] The kit and method of the present invention will be described in more detail below by taking the study of differences in dendritic cell typing, differentiation and function between AIDS patients and healthy people as an example. However, the examples given are only for explaining the present invention, not for limiting the scope of the present invention. The kit and method of the present invention can also be used in the research of other immune-related diseases, and clinically evaluate the curative effect of immunotherapy.

[0033] Example Using the kit and method of the present invention to study the differences between AIDS patients and healthy people in the subgroups, degree of differentiation and aspects of dendritic cells

[0034] 1. Experimental steps

[0035] 1) Pretreatment of peripheral blood of AIDS patients

[0036] Take 1 drop (50-100 μl) of peripheral blood from the ear veins of AIDS patients and healthy adults, and carry out anticoagulant treatment; mix...

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Abstract

The invention relates to a kit for combining and analyzing a CD11c+CD11b+DC subgroup and differentiation degree and functions thereof. The kit comprises eight antibodies: CD11c, CD80, CD86, CD11b, HLA-RD, IL-12, IL-23 and IL-27. The invention further provides a method for combining and analyzing a CD11c+CD11b+dendritic cell sub-group and the differentiation degree and functions thereof. With the adoption of the kit and the method, a whole set of data of the CD11c+CD11b+dendritic cell subgroup and the differentiation degree and functions thereof can be detected in one step and similar data does not need to be additionally and tediously tested respectively. By applying a design scheme of the invention, the ratio quantity of a CD11c+CD11b+subgroup in dendritic cells can be obtained and the development, differentiation and mature conditions of the subgroup can also be known; and a function condition of the subgroup can also be known and the effect of rapidly and simply obtaining a whole set of immunology detection data of the CD11c+CD11b+dendritic cell subgroup in one step is realized.

Description

technical field [0001] The present invention relates to the field of immunological detection, more particularly to a kit and method for combined analysis of dendritic cell subsets, degree of differentiation and function Background technique [0002] Flow cytometry is a device for automatic analysis and sorting of cells. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can identify or sort specified cell subpopulations according to pre-selected parameter ranges. [0003] In actual use, the instrument first measures the light scattering signal. When light scattering analysis is used in conjunction with fluorescent probes, stained and unstained cells can be identified in a sample. The most effective use of light scattering measurements is to identify certain subpopulations from heterogeneous populations. [0004] The fluorescent signal mainly includes two parts: ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/56966G01N33/68G01N33/6869
Inventor 张学峰周放欧阳坤刘光伟
Owner YEACELL BIOTECH INC
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