Soil conditioner
A technology of soil conditioner and activator, which is applied in the direction of soil conditioning materials, organic fertilizers, fertilizer mixtures, etc. It can solve the problems of long-term action of microbial conditioners, difficult removal of chemical reagents, and high potential risks, and achieve a strong soil base Exchange ability, solve the problem of secondary pollution, good effect
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[0049] Example 1
[0050] This embodiment provides a microbial inoculum and a preparation method thereof. The method includes the following steps:
[0051] The Bacillus subtilis strain was purchased from the Agricultural Microbiology Center of the China Microbial Culture Collection Management Committee, the deposit number is ACCC11025, the genus name: Bacillus, and the species name: subtilis.
[0052] 1), strain activation
[0053] Inoculating the low-temperature stored Bacillus subtilis strain into LB liquid medium, shaking culture at 30°C for 20 to 24 hours, and using the obtained fermentation broth as the inoculum;
[0054] 2) Preparation of seed liquid
[0055] Connect the inoculum prepared in step 1) to the LB liquid medium at a ratio of 2% to 5% by volume, and shake culture at 30° C. for 12 to 16 hours to obtain a seed solution.
[0056] 3), fermentation culture
[0057] The seed liquid and the fermentation medium obtained in step 2) are connected to a fermentor containing a fermenta...
Example Embodiment
[0061] Example 2
[0062] This embodiment provides a soil conditioner and a preparation method thereof. The soil conditioner includes the following components:
[0063] Mineral materials, activator A, the microbial inoculum provided in Example 1, activator B for providing nutrition to the microbial inoculum, and enzyme preparation;
[0064] The main components of the enzyme preparation are cellulase and urease in equal mass ratio;
[0065] The mineral material includes 100-200 mesh attapulgite, sepiolite, and zeolite; the mass ratio of the attapulgite, sepiolite, and zeolite is 0.8:0.6:0.8;
[0066] The ingredients of the activator A are a mixture of citric acid, aminoacetonitrile hydrogen sulfate and chelated zinc and boron in a mass ratio of 1:0.01:0.06;
[0067] The mass percentage of the enzyme preparation in the mineral material is 0.05%;
[0068] The activator B includes yeast extract, peptone and amino acids; the mass ratio of the yeast extract, peptone and amino acids is 0.8:1.8:0...
Example Embodiment
[0073] Example 3
[0074] This embodiment provides a soil conditioner and a preparation method thereof. The soil conditioner includes the following components:
[0075] Mineral materials, activator A, the microbial inoculum provided in Example 1, activator B for providing nutrition to the microbial inoculum, and enzyme preparation;
[0076] The main components of the enzyme preparation are cellulase and urease in equal mass ratio;
[0077] The mineral material includes 100-200 mesh attapulgite, sepiolite, and zeolite; the mass ratio of the attapulgite, sepiolite, and zeolite is 1.2:0.4:1.2;
[0078] The composition of the activator A is a mixture of citric acid, aminoacetonitrile hydrogen sulfate and chelated zinc and boron in a mass ratio of 1:0.03:0.04;
[0079] The mass percentage of the enzyme preparation in the mineral material is 0.1%;
[0080] The activator B includes yeast extract, peptone and amino acids; the mass ratio of the yeast extract, peptone and amino acids is 1.2:2.2:1.2...
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