Universal primer set for magnifying long segments of mitochondrial genomes of liriomyza mik insects

A universal primer and mitochondrial technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial measurement / inspection, etc., to achieve the effect of shortening the experimental cycle, shortening the acquisition cycle, and reducing the cost of the experiment

Inactive Publication Date: 2016-09-21
YANGZHOU UNIV
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Problems solved by technology

At present, there is no relevant report on universal primers for the amplification of long fragments of the mitochondrial genome of Liriomyza insects

Method used

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  • Universal primer set for magnifying long segments of mitochondrial genomes of liriomyza mik insects
  • Universal primer set for magnifying long segments of mitochondrial genomes of liriomyza mik insects
  • Universal primer set for magnifying long segments of mitochondrial genomes of liriomyza mik insects

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Embodiment Construction

[0013] The method for screening primers for amplification of long fragments of Liriomyza mitochondrial genome of the present invention is divided into two steps: 1. According to the principle of primer design, by comparing the measured hexapod mtDNA sequences, as far as possible in the conserved region Design the primers, and consider the introduction of degenerate sites to increase the versatility of the primers. Finally, design 2 pairs of long PCR primers to amplify 2 large fragments that overlap each other and cover the entire mitochondrial genome through long PCR; 2. The synthesized primers The mitochondrial genome of Liriomyza insects was amplified, and its generality and amplification efficiency in Liriomyza were tested.

[0014] Below in conjunction with example the invention is further described:

[0015] 1. Sample preparation: The samples were all insects of different species of Liriomyza genus stored in 100% alcohol in the specimen room of the Institute of Applied En...

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Abstract

The invention discloses a universal primer set for magnifying long segments of mitochondrial genomes of liriomyza mik insects. The primer set consists of two pairs of primers, of which sequences are shown by SEQ ID NO.1 TO SEQ ID NO. 4. According to the magnification primer set, long segments of mitochondrial genomes of multiple liriomyza mik species can be effectively magnified, the molecular data acquisition cycle can be favorably shortened, and an important tool is provided for phylogenic study and molecular identification specific primer loci of the liriomyza mik insects.

Description

technical field [0001] The invention relates to two pairs of universal primers for amplifying long fragments of the mitochondrial genome of Liriomyza insects. Background technique [0002] The genus Liriomyza Mik belongs to Diptera, Agromyzidae, and Phtomyzinae, and is a worldwide pest that harms vegetables and flowers. Liriomyza sativus has a wide range of hosts, and both adults and larvae can cause damage to crops. The larvae feed on the leaves or petioles of host plants, seriously affecting the photosynthesis of host plants, causing the host plants to lose leaves and flowers, delay development and die. Feeding, laying eggs, plant damage wounds also provide a way for pathogens to invade. According to domestic and foreign literature records and statistics from local censuses, there are currently 19 species of pests in this genus that are distributed in my country (including Taiwan and Hong Kong Special Administrative Region of my country), including four typical polyphagou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10
CPCC12N15/11C12Q1/686C12Q2531/113
Inventor 杜予州常亚文
Owner YANGZHOU UNIV
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