Preparation method for total flavonoids in V.amoena Fisch.var.angusta Freyn.

A technology of vetch and total flavonoids, applied to medical preparations containing active ingredients, pharmaceutical formulas, non-central analgesics, etc., can solve the problem of low extraction rate, achieve high extraction rate, high physical stability, and adsorption good selectivity

Active Publication Date: 2016-10-12
LIAONING UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the extraction methods of total flavonoids mainly include pH gradient extraction method, ethyl acetate extraction method, alkali extraction and acid precipitation method, and the extraction rate is low

Method used

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  • Preparation method for total flavonoids in V.amoena Fisch.var.angusta Freyn.
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  • Preparation method for total flavonoids in V.amoena Fisch.var.angusta Freyn.

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Experimental program
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Effect test

Embodiment 1

[0012] The preparation method of embodiment 1 total flavonoids of wild pea

[0013] 1) Extraction: take 10 g of the medicinal material powder of Vetchia sayama, 200 mL of 70% ethanol aqueous solution (the ratio of liquid to material is 1:20), and the extraction temperature is 90 ° C, and the extraction is performed twice, and the extraction time is 1 hour each time to obtain the extract;

[0014] 2) Purification: Select an adsorption column (Φ1.5cm×20cm, 10g) filled with HPD-826 macroporous adsorption resin, and rotatively evaporate the extract obtained in step 1) to recover ethanol to obtain a concentrated solution, and add water to the concentrated solution to adjust the concentration 0.39mg / mL, use HCl to adjust the pH of the sample aqueous solution to 5.5, the loading volume is 5 times the volume of the resin column bed, and the flow rate is adjusted to 2mL / min for dynamic adsorption. The eluent is 70% ethanol aqueous solution, and the flow rate is 3mL / min Carry out elutio...

Embodiment 2

[0016] The optimization of embodiment 2 extraction steps

[0017] (1) The influence of extraction solvent

[0018] Accurately weigh 5g of the wild pea medicinal material powder (No. 4 sieve), totally 7 parts, put in a 250ml Erlenmeyer flask, add 40%, 50%, 60%, 70%, 80%, 95% ethanol aqueous solution 75ml respectively (material The liquid ratio is 1:15), heated at 90°C for 1 hour, and extracted twice. The extracts were combined, evaporated to dryness, dissolved in methanol, and adjusted to 50ml. Determination of the content of total flavonoids in the extract, the results are shown in Table 1.

[0019] Table 1 Extraction Reagent Investigation Results

[0020]

[0021] (2) The influence of extraction conditions

[0022] The extraction solvent concentration is 70% ethanol aqueous solution. Orthogonal experimental design was carried out with four factors: extraction temperature, solid-liquid ratio, extraction time, and extraction times. The factor levels are shown in Table 2,...

Embodiment 3

[0030] The optimization of embodiment 3 purification steps

[0031] (1) The effect of macroporous adsorption resin on the total flavonoids of Sayama vetch

[0032] The resin models investigated are HPD-750, HPD-600, HPD-826, HPD-5000, ADS-17, ADS-7, AB-8, D101, X-5, DM-130. Accurately weigh 1 g of each of the 10 pretreated macroporous resins (dry and anhydrous), place them in a 50 mL Erlenmeyer flask, and add 50 mL of crude drug concentration of 0.17 g / mL solution (5 kinds of flavonoid glycoside concentration C 0 0.6404mg / mL), shake constantly (120r / min), shake for 2 hours, filter, take the filtrate, measure the content of 5 flavonoid glycosides, and get the concentration C 1 . Take the 10 kinds of macroporous resins obtained by the above filtration, place them in 50mL conical flasks respectively, add 50mL70% ethanol aqueous solution respectively, shake from time to time (120r / min), shake for 2 hours, filter, take the subsequent filtrate, measure The content of 5 flavonoid ...

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Abstract

The invention relates to a preparation method for total flavonoids in V.amoena Fisch.var.angusta Freyn.. According to the technical scheme, V.amoena Fisch.var.angusta Freyn. medicinal material powder is taken and extracted at 70-90 DEG C with a 40-95% ethanol water solution as the solvent in the liquid-material ratio of 1:(10-20), and extraction liquor is obtained; an adsorption column filled with weak polarity-medium polarity macroporous adsorption resin is selected, the total flavonoid loading concentration is 0.21-3.18 mg / mL, the pH is regulated to be 1-8, samples are loaded on the adsorption column, the loading volume is 4-6 times the resin column bed volume, the adsorption flow velocity is regulated to be 2-6 mL / min for dynamic adsorption, elution is carried out with a 30-95% ethanol water solution at the flow velocity of 2-6 mL / min, and elution liquor is collected. According to the macroporous adsorption resin preparation method for total flavonoids, the yield of total flavonoids is high, desorption conditions are mild, the service cycle is long, regeneration is easy and convenient, the extraction ratio is high, and the cost is low; the preparation method is suitable for popularization and application in industrial production.

Description

technical field [0001] The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a preparation method of the effective part of the traditional Chinese medicine of wild pea. Background technique [0002] Narrow mountain vetch (V.amoena Fisch.var.angusta Freyn.) is a plant of the leguminosae genus ViciaL. It is mainly distributed in Northeast my country, North China, Northwest China and other places. Used as medicine. The prescriptions of the Chinese patent medicines "Kanlisha", "Yangxue Rongjin Pills" and "Shutong'an Tubing" recorded in the first part of the "Chinese Pharmacopoeia" in the 2015 edition all contain S. In the preparation, the medicinal materials and decoction pieces not included in this edition of the Pharmacopoeia) included V.amoena Fisch.var.angusta Freyn. as Sperantosporum. [0003] Sayama vetch has "sweet taste and flat nature", "can nourish, harmonize, and relieve", it belongs to the liver and kidney ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P29/00A61P37/02
CPCA61K36/48A61K2236/333A61K2236/55
Inventor 桑育黎郝延军郭方岩陈长兰陈立江刘玉峰徐亮孔玉梅王宇
Owner LIAONING UNIVERSITY
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