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Preparation method of total flavonoids in Sayama vetch

A technology of vetch and total flavonoids, applied to medical preparations containing active ingredients, pharmaceutical formulas, non-central analgesics, etc., can solve the problem of low extraction rate

Active Publication Date: 2021-04-09
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the extraction methods of total flavonoids mainly include pH gradient extraction method, ethyl acetate extraction method, alkali extraction and acid precipitation method, and the extraction rate is low

Method used

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  • Preparation method of total flavonoids in Sayama vetch
  • Preparation method of total flavonoids in Sayama vetch
  • Preparation method of total flavonoids in Sayama vetch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The preparation method of embodiment 1 total flavonoids of wild pea

[0013] 1) Extraction: take 10 g of the medicinal material powder of Vetchia sayama, 200 mL of 70% ethanol aqueous solution (the ratio of liquid to material is 1:20), and the extraction temperature is 90 ° C, and the extraction is performed twice, and the extraction time is 1 hour each time to obtain the extract;

[0014] 2) Purification: Select an adsorption column (Φ1.5cm×20cm, 10g) filled with HPD-826 macroporous adsorption resin, and rotatively evaporate the extract obtained in step 1) to recover ethanol to obtain a concentrated solution, and add water to the concentrated solution to adjust the concentration 0.39mg / mL, use HCl to adjust the pH of the sample aqueous solution to 5.5, the loading volume is 5 times the volume of the resin column bed, and the flow rate is adjusted to 2mL / min for dynamic adsorption. The eluent is 70% ethanol aqueous solution, and the flow rate is 3mL / min Carry out elutio...

Embodiment 2

[0016] The optimization of embodiment 2 extraction steps

[0017] (1) The influence of extraction solvent

[0018] Accurately weigh 5g of the wild pea medicinal material powder (No. 4 sieve), totally 7 parts, put in a 250ml Erlenmeyer flask, add 40%, 50%, 60%, 70%, 80%, 95% ethanol aqueous solution 75ml respectively (material The liquid ratio is 1:15), heated at 90°C for 1 hour, and extracted twice. The extracts were combined, evaporated to dryness, dissolved in methanol, and adjusted to 50ml. Determination of the content of total flavonoids in the extract, the results are shown in Table 1.

[0019] Table 1 Extraction Reagent Investigation Results

[0020]

[0021] (2) The influence of extraction conditions

[0022] The extraction solvent concentration is 70% ethanol aqueous solution. Orthogonal experimental design was carried out with four factors: extraction temperature, solid-liquid ratio, extraction time, and extraction times. The factor levels are shown in Table 2,...

Embodiment 3

[0030] The optimization of embodiment 3 purification steps

[0031] (1) The effect of macroporous adsorption resin on the total flavonoids of Sayama vetch

[0032] The resin models investigated are HPD-750, HPD-600, HPD-826, HPD-5000, ADS-17, ADS-7, AB-8, D101, X-5, DM-130. Accurately weigh 1 g of each of the 10 pretreated macroporous resins (dry and anhydrous), place them in a 50 mL Erlenmeyer flask, and add 50 mL of crude drug concentration of 0.17 g / mL solution (5 kinds of flavonoid glycoside concentration C 0 0.6404mg / mL), shake constantly (120r / min), shake for 2 hours, filter, take the filtrate, measure the content of 5 flavonoid glycosides, and get the concentration C 1 . Take the 10 kinds of macroporous resins obtained by the above filtration, place them in 50mL conical flasks respectively, add 50mL70% ethanol aqueous solution respectively, shake from time to time (120r / min), shake for 2 hours, filter, take the subsequent filtrate, measure The content of 5 flavonoid ...

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Abstract

The invention relates to a preparation method of total flavonoids in Sayama vetch. The technical scheme adopted is: take the medicinal material powder of the wild pea from the mountain, use 40-95% ethanol aqueous solution as a solvent, and extract at a liquid-to-material ratio of 1:10-20 at 70-90° C. to obtain an extract. Select an adsorption column filled with weakly polar-medium polar macroporous adsorption resin, the concentration of total flavonoids in the sample is 0.21-3.18mg / mL, adjust the pH to 1-8, and load the sample on the adsorption column with a sample volume of 4- 6 times the volume of the resin column bed, adjust the adsorption flow rate to 2-6mL / min for dynamic adsorption, use 30-95% ethanol aqueous solution, 2-6mL / min flow rate for elution, and collect the eluate. The preparation method of the total flavone macroporous adsorption resin of the present invention has high yield of total flavonoids, relatively mild desorption conditions, long service life, simple regeneration, high extraction rate and low cost, and is suitable for popularization and application of industrial production.

Description

technical field [0001] The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a preparation method of the effective part of the traditional Chinese medicine of wild pea. Background technique [0002] Narrow mountain vetch (V.amoena Fisch.var.angusta Freyn.) is a leguminosae (Leguminosae) plant of the genus Vicia (Vicia L.). It is mainly distributed in Northeast my country, North China, Northwest China and other places. Herbs are used as medicine. The prescriptions of the Chinese patent medicines "Kanlisha", "Yangxue Rongjin Pills" and "Shutong'an Tubing" recorded in the first part of the "Chinese Pharmacopoeia" in the 2015 edition all contain S. In the preparation, the medicinal materials and decoction pieces not included in this edition of the Pharmacopoeia) included V.amoena Fisch.var.angusta Freyn. as Sperantosporum. [0003] Sayama vetch has "sweet taste and flat nature", "can nourish, harmonize, and relieve", it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P29/00A61P37/02
CPCA61K36/48A61K2236/333A61K2236/55
Inventor 桑育黎郝延军郭方岩陈长兰陈立江刘玉峰徐亮孔玉梅王宇
Owner LIAONING UNIVERSITY
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