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A method for monitoring the precipitation process of human serum albumin acetate buffer based on near-infrared spectroscopy

A technology of acetate buffer and human serum albumin, which is applied in the direction of material analysis, analysis material, measuring device, etc. by optical means to achieve the effect of improving production efficiency

Active Publication Date: 2018-11-30
SHANDONG UNIV
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Problems solved by technology

However, there are no reports on the monitoring of the precipitation process of human serum albumin acetate buffer or the establishment of related models by near-infrared spectroscopy analysis technology at home and abroad.

Method used

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  • A method for monitoring the precipitation process of human serum albumin acetate buffer based on near-infrared spectroscopy
  • A method for monitoring the precipitation process of human serum albumin acetate buffer based on near-infrared spectroscopy
  • A method for monitoring the precipitation process of human serum albumin acetate buffer based on near-infrared spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the establishment of the assay model for HA content

[0042](1) Acetate buffer precipitation process: take 100mL of FIV supernatant after pressure filtration (provided by Shandong Taibang Biological Products Co., Ltd.) and place it in a round-bottomed flask, and then place the round-bottomed flask in a low-temperature reactor with a constant temperature; the process Take a sample of 1 mL before the start and then start to add acetate buffer dropwise. The method of adding acetate buffer is 0.2 mL each time for the first 9 times, and 0.8 mL each time for the last 6 times; during the process, add acetate buffer every 4 minutes and sample 1 mL. Do centrifugation and repeat 8 batches in total. Acquisition of near-infrared spectra: The transmission module of the Antaris II Fourier transform near-infrared spectrometer collects the original near-infrared spectra. Optical path 4mm, spectral range 10000-4000cm -1 , with a resolution of 8cm -1 , the number of scan...

Embodiment 2

[0052] Embodiment 2: be used for the establishment of pH value determination model

[0053] (1) Acetate buffer precipitation process: Simulate the precipitation process of 6 batches of acetate buffer, except for the 6th batch, the steps of the other 5 batches of acid precipitation are consistent with the process in Example 1 (1). In the sixth batch, 1 ml of ethanol was added each time after acid addition for 13 times, a total of 2 additions, and the rest of the conditions were consistent with those of the first 5 batches. Each time 1.2ml was sampled and centrifuged. The original near-infrared spectrum collection conditions are the same as in Example 1 (1), the ethanol content of the sample is measured by gas chromatography, and a sample with 10% (volume fraction) of ethanol is prepared according to the content, and the pH meter measures the pH value of the sample. The pH values ​​of 74 samples were obtained for the establishment of quantitative models. Table 4 is the informat...

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Abstract

The invention discloses a method for near infrared spectral analysis technology-based monitoring of a human albumin acetic acid buffer deposition process. The method comprises building a model for determining HA content in a human albumin acetic acid buffer deposition process through a near infrared spectral analysis technology, building a model for pH determination and carrying out process determination on HA content and pH in a human albumin acetic acid buffer deposition process through the models. The method can realize process determination of HA content and pH in a human albumin acetic acid buffer deposition process and can timely adjust an acetic acid buffer adding rate and accurately and timely determine a reaction terminal point according to HA content change.

Description

technical field [0001] The invention relates to a method for monitoring the precipitation process of human serum albumin acetate buffer based on near-infrared spectrum analysis technology. Background technique [0002] Human albumin (HA) is the protein with the highest content in human plasma, accounting for 40%-60% of plasma protein. HA is clinically used to treat various diseases, including hypovolaemia, hypoalbuminemia, shock, burns, surgical blood loss, trauma, chronic liver disease, nutritional support, and resuscitation of critically ill patients. Since HA is mostly isolated from human plasma, the source of raw materials is scarce, and the supply is often in short supply in the market. [0003] At present, the production of HA is basically separated from human plasma by the low-temperature ethanol method. Due to the huge amount of plasma input in the industrial production of HA (generally measured in tons), the volume of the reaction tank is also large, and the huge v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359G01N21/3577
CPCG01N21/3577G01N21/359G01N2021/3595
Inventor 臧恒昌庞广礼王斐仲立军李灿张惠王佳月范加金王鸣宇
Owner SHANDONG UNIV
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