Fractionation device for carrying out simple and rapid ion exchange on peptide or protein mixture

Abstract

The invention provides a fractionation device for carrying out simple and rapid ion exchange on a peptide or protein mixture. The fractionation device is low in manufacturing cost, while can obtain the same fractionation effect as a corresponding commercial product. The device comprises a component A, a component B and a component C. The component B is a chromatographic column, wherein A guide pipe penetrates through the centers of two hollow screws, the guide pipe stretches into a two-way parts on the left and right sides, the left and right ends of the two-way parts are provided with internal threads, the two hollow screws are provided with external threads and are in threaded connection with the left and right two-way parts, and sealing iron loops and screen plates are sequentially arranged in front of the two hollow screws respectively. According to the component A, a guide pipe penetrates through the centers of the two hollow screws, one screw is in threaded connection with a two-way part on the left side of the component B, and a sealing iron loop is arranged in front of the screw of the component A. According to the component C, a hollow screw is in threaded connection with a two-way part in the component B through a sealing iron loop, and then a capillary tube for collecting fraction is inserted into the hollow screw of the component C.

Description

technical field [0001] The invention relates to proteomics in the field of life sciences, in particular to the technical field of high performance liquid chromatography. Background technique [0002] Qualitative and quantitative analysis of complex peptides and protein mixtures is currently mainly done by the instrumental analysis method of multidimensional high performance liquid chromatography-mass spectrometry. Multidimensional high performance liquid chromatography is mainly composed of ion-exchange chromatography and reversed-phase chromatography. Reversed-phase chromatography is generally used in the last one-dimensional and Mass spectrometry is used in conjunction to realize online separation and analysis; ion exchange chromatography is generally used for rough pre-fractionation of original mixture samples. However, commercial ion-exchange HPLC systems are often expensive, with prices in the $100,000 range. Contents of the invention [0003] In order to solve the ...

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Problems solved by technology

[0003] In order to solve the aforementioned problem of high price of commercial ion-exchange high-performance liquid chromatography systems, the present invention provides a simple and fast ion-exchange fractionation device for polypeptide or protein mixtures that can achieve the same fractionation effect as the corresponding commercial products with low manufacturing cost , including A part, B part and C part, the B part is a chromatographic column, a conduit runs through the center of two hollow screws, and the conduit extends into the two links on the left and right sides, and the left and right ends of the two links have Internal threads, two hollow screws have external threads, which are respectively connected to the left and right two-way joints, and the front ends of the two hollow screws are successively provided with a sealing iron ferrule and a sieve plate; the A part also runs through the center of the two hollow screws A conduit, one of the screws is connected to the left two-way screw of part B, and the front of the screw of part A has a sealing iron ferrule; the C part uses a hollow screw to pass through the sealing iron ferrule and the two-way screw in part B. Then use a capillary for collecting fractions and insert it into the hollow screw in part C

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