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A kind of preparation method of fucoidan

A technology of fucoidan and brown algae, which is applied in the field of comprehensive utilization of brown algae to extract fucoidan, can solve the problems of in-depth application research, high product cost, and difficult market development, so as to increase iodine yield, reduce burden, and ensure stability Effect

Active Publication Date: 2018-12-21
青岛明月海琳岩藻多糖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the raw materials for producing fucoidan are mainly brown algae such as kelp, but brown algae resources are limited, not only to provide more and more daily food, but also a large number of brown algae enter the alginate production industry, which can meet the raw materials for fucoidan production It is very limited, and the fucoidan content in most brown algae is only about 1%. If brown algae are used only for the production of fucoidan, it will not only cause a great waste of brown algae resources, but also directly lead to high product costs. Difficult to develop

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  • A kind of preparation method of fucoidan

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preparation example Construction

[0024] The preparation method of fucoidan provided in this example is prepared by using brown algae washing water alkali slag as raw material, utilizing the characteristics of fucoidan to be precipitated under alkaline conditions and dissolved under acidic conditions, and prepared through complex extraction processes. The quality of fucoidan products is stable, which overcomes the shortcomings of low yield and high price of fucoidan extraction.

[0025] The preparation method is specifically:

[0026] 1) Washing brown algae, the brown algae is kelp, hijiki, kelp or wakame. Add 8-10 times the weight of brown algae to wash with water, the washing temperature is 45-55°C, and stir for 2-3 hours. The clean brown algae enters the algin production process, and the washing water is retained. Add alkali to the washing water to adjust to pH=10-11, and the alkali used is one or more of sodium hydroxide, potassium hydroxide, and calcium hydroxide; foam and float the alkali slag, let it ...

Embodiment 1

[0040] (1) Add 8-10 times the quality of water to 5kg of dried kelp, stir and wash at 50°C for 4 hours, add sodium hydroxide to the washing water to adjust the pH=10-11, stir well, settle on a slant plate, and take the upper layer of floating alkali slag 6kg of liquid, 4kg of supernatant obtained through decanter centrifuge is used for recovery and iodine extraction, and 2kg of alkali slag is collected to enter the fucoidan production process.

[0041] (2) Add acetic acid to 2 kg of alkali slag to adjust pH=4-4.5, stir to dissolve, and remove insoluble impurities through disc centrifugation to obtain 3 kg of solution.

[0042] (3) Add 3 g of protease, perform enzymatic hydrolysis at 45-55°C for 2 hours, and inactivate the enzyme at 90°C for 30 minutes after enzymatic hydrolysis.

[0043] (4) The enzymolysis solution enters the concentration process and is concentrated to about 20% solid content. There is about 0.7kg of concentrated solution, and 0.233L of absolute ethanol is a...

Embodiment 2

[0046] (1) Add 8-10 times the quality of water to 1t of undaria pinnatifida, stir and wash at 50°C for 4 hours, add potassium hydroxide to the washing water to adjust the pH=10-11, stir well, settle on an inclined plate, and take 1200kg of floating slag from the upper layer, 800kg of supernatant obtained through the decanter centrifuge is used to recover iodine, and 400kg of alkali smelting slag enters the fucoidan production process.

[0047] (2) Add hydrochloric acid to 400 kg of alkali slag to adjust pH=4-4.5, stir to dissolve, and remove insoluble impurities through disc centrifugation to obtain 600 kg of solution.

[0048] (3) Add 600 g of protease, perform enzymatic hydrolysis at 45-55°C for 2 hours, and inactivate the enzyme at 90°C for 30 minutes after enzymatic hydrolysis.

[0049] (4) The enzymolysis solution enters the concentration process and is concentrated until the solid content is about 20%. There is about 140kg of concentrated solution, and 46.6L of absolute ...

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Abstract

The invention discloses a method for preparing fucoidin that is prepared by extracting with brown alga washing water as raw material. The method comprises the steps of 1), washing brown algae, adding an alkali to the washing water, foaming floating alkali refined residue, and collecting upper floating alkali refined residue and part of clear liquid; 2), subjecting alkali refined residue liquid to solid-liquid separation to obtain the clear liquid and alkali refined solid residue; 3), adding a dilute acid to the alkali refined solid residue, soaking, centrifuging, and collecting supernate; 4), enzymatically hydrolyzing the supernate, and enzymatically deactivating to obtain an enzymatic hydrolysate; 5), concentrating the enzymatic hydrolysate under reduced pressure, performing alcoholic precipitation, and collecting precipitate; 6), rinsing the precipitate with a decolorant, drying and removing the decolorant, adding water for dissolving, adding activated carbon, collecting upper concentrate, finely filtering, and drying to obtain fucoidin. The brown alga washing water in extraction preprocessing process of alginate is used to extract fucoidin, brown alga resources are not used independently, and the brown alga resources are comprehensively utilized.

Description

technical field [0001] The invention belongs to the technical field of seaweed chemical industry, and in particular relates to a method for comprehensively utilizing brown algae to extract fucoidan. Background technique [0002] Fucoidan (FUC) is a class of polysaccharides mainly composed of fucose and sulfate groups, widely present in brown algae and some marine invertebrates (such as sea cucumbers, sea urchins, etc.), and has a variety of Physiological activities, such as anticoagulant and antithrombotic, antiviral, antitumor, immune regulation, anti-inflammatory, hypolipidemic, antioxidative, anti-complement properties, gastric protection, etc., in recent years, for the purpose of drug and health food development There are more and more studies on FUC. [0003] Brown algae rich in fucoidan include mozuku, kelp, kelp, wakame, etc. Fucoidan is a sticky substance secreted from the surface of the algae, which keeps the leaves moist and prevents microbial invasion, acting as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王春霞王晓梅姜进举尹宗美李可昌范素琴王正平
Owner 青岛明月海琳岩藻多糖生物科技有限公司